东南园艺2025，Vol.13Issue(6)：573-581,9.DOI:10.20023/j.cnki.2095-5774.2025.06.004
珍稀兰科植物掌裂兰的非共生萌发研究
Study on the asymbiotic germination of rare Orchidaceae plant Dactylorhiza hatagirea
摘要
Abstract
[Objective]Dactylorhiza hatagirea is an orchid plant used both as medicine and food in Linzhi City,Tibet Autonomous Region.To explore the effects of different influencing factors on the asymbiotic germination of Dactylorhiza hatagirea seeds.[Method]This experiment took the asymbiotic germination rate of Dactylorhiza hatagirea seeds as an indicator to investigate the effects of different basic media(MS,N6,and Hyponex No.1),different concentrations of exogenous hormones(NAA and 6-BA),different seed development times,and different concentrations of activated carbon and vitamins on the germination of Dactylorhiza hatagirea seeds.[Result]The results showed that:1.In terms of basic media,there was little difference in the germination rate of Dactylorhiza hatagirea seeds among MS,N6,and Hyponex No.1 basic media.2.Regarding single exogenous hormones at different concentrations,the germination rate of Dactylorhiza hatagirea seeds with the addition of NAA and 6-BA at any concentration was lower than that without the addition of exogenous hormones.3.In terms of seed development time,the germination rate of Dactylorhiza hatagirea seeds first increased and then decreased with the increase of development time.4.With respect to different additives,the germination rate of Dactylorhiza hatagirea seeds without the addition of activated carbon or vitamin C was 0%.The addition of activated carbon and vitamin C at different concentrations could improve the germination rate,but there were certain differences in the subsequent growth and development of protocorms.[Conclusion]The three basic media,namely 1,MS,N6,and Huabao No.1,have little impact on the seed germination of Dactylorhiza hatagirea.The exogenous hormones NAA and 6-BA have an inhibitory effect on the asymbiotic germination of Dactylorhiza hatagirea seeds.The germination rate of Dactylorhiza hatagirea seeds 60 days after pollination is the highest,while the seeds 30 days after pollination have no germination ability.2 g/L activated carbon and 0.5 g/L vitamins have the strongest stimulating effect on the embryos of Dactylorhiza hatagirea seeds whose activity has decreased after being stored in a refrigerator at-25℃for 6 months,resulting in the highest germination rate.The protocorms germinated from Dactylorhiza hatagirea seeds with activated carbon added develop better than those with vitamins added.关键词
掌裂兰/基础培养基/外源激素/发育时间/其他添加物/非共生萌发/兰科植物/药食同源Key words
Dactylorhiza hatagirea/basic culture medium/exogenous hormone/development time/other additives/asymbiotic germination/Orchidaceae/medicinal and edible homology分类
农业科技引用本文复制引用
刘念,章容杰,谢军,白央,陈学达,邢震..珍稀兰科植物掌裂兰的非共生萌发研究[J].东南园艺,2025,13(6):573-581,9.基金项目
林芝市林业和草原局重点项目(LZLCJ603324061) （LZLCJ603324061）
西藏自治区教育厅项目(XK2024-01、XK2024-04、YJSJD2025-6) （XK2024-01、XK2024-04、YJSJD2025-6）
西藏农牧学院林学博士点(一期)(533325001) （一期）
