分析测试学报2026，Vol.45Issue(2)：452-460,9.DOI:10.12452/j.fxcsxb.25091104
基于RNA切割型DNAzyme的肿瘤标志物检测技术研究进展
Advances in Tumor Marker Detection Technology Based on RNA-cleaving DNAzyme
摘要
Abstract
Tumor markers are biologically active substances abnormally produced or released by tu-mor cells or the host body in response to the presence of a tumor.These primarily include proteins,nucleic acids,circulating tumor cells(CTCs),small extracellular vesicles(sEVs),and metabo-lites,which can be detected in blood,body fluids,or tissues.The tumor markers typically exist at low concentrations,particularly during the early stages of cancer development.Therefore,highly sensitive and accurate detection technologies play a significant role in the early screening,therapeu-tic effect monitoring and prognosis assessment,recurrence warning,and precision medicine of can-cer.RNA-cleaving DNAzyme is a bifunctional nuclease with both molecular recognition and catalytic cleavage.This unique property makes them highly promising for the precise detection of trace or ultra-trace levels of tumor biomarkers.This article provides a systematic review of the working principles of RNA-cleaving DNAzyme,with a focused analysis of their applications and recent advances in tumor biomarker detection.Furthermore,it comprehensively summarizes research progress in multiplexed tumor marker detection using RNA-cleaving DNAzyme,particularly highlighting their integration with microfluidic chips,microarray platforms,and point-of-care testing(POCT)systems.Finally,the review offers perspectives on future research directions and potential clinical applications of these catalytic nucleic acids.关键词
RNA切割型DNAzyme/肿瘤标志物/检测/核酸催化/分子识别Key words
RNA-cleaving DNAzyme/tumor marker/detection/nucleic acid catalysis/molecu-lar recognition分类
化学化工引用本文复制引用
胥欣悦,李健慧,李玉娇,由田田,吴拥军,刘利娥,丁丽华..基于RNA切割型DNAzyme的肿瘤标志物检测技术研究进展[J].分析测试学报,2026,45(2):452-460,9.基金项目
国家自然科学基金青年项目(82304194) （82304194）
河南省重点研发与推广专项(科技攻关)(232102311010,242102311174) （科技攻关）
河南省大学生创新训练项目(S202510459170) （S202510459170）
