分析化学2026，Vol.54Issue(1)：50-59,10.DOI:10.19756/j.issn.0253-3820.251240
硫磺素T非标记荧光核酸适配体传感器快速检测二氯喹啉酸除草剂
A Thioflavin T-based Label-Free Fluorescent Aptasensor for Rapid Detection of Quinclorac Herbicide
摘要
Abstract
A label-free fluorescent aptasensor based on thioflavin T(ThT)fluorescent probe was developed for rapid detection of quinclorac(QNC).In this sensor system,ThT specifically bound to the QNC aptamer Qapt-48nt with G-quadruplex structure,resulting in a significantly enhanced fluorescence signal.Upon addition of QNC,it competitively bound to Qapt-48nt,forming a QNC-Qapt complex and releasing free ThT,leading to a decrease in the fluorescence intensity of the system.Under optimal conditions,the linear detection range of this sensor for QNC was 0.012–4.84 μg/mL,the limit of detection(S/N=3)was 3.3 ng/mL,and the limit of quantification(S/N=10)was 9.8 ng/mL.This method was successfully applied to detection of QNC in river water,soil and rice samples,with recoveries ranging from 83.3%to 108.3% and relative standard deviations of 3.9% to 8.8%,and the results were comparable to those of HPLC-MS/MS method.The established aptasensor could complete the detection of QNC within 2 min,featuring simplicity,rapidity,and high specificity,and providing a novel method for rapid detection of QNC residues in environmental and food samples.关键词
核酸适配体/荧光/二氯喹啉酸/硫磺素T/传感器Key words
Aptamer/Fluorescence/Quinclorac/Thioflavin T/Sensor引用本文复制引用
方灵,罗桂珍,黄智鸿,王宗文..硫磺素T非标记荧光核酸适配体传感器快速检测二氯喹啉酸除草剂[J].分析化学,2026,54(1):50-59,10.基金项目
福建省属公益项目(No.2022R1022001)和福建省农产品质量安全重点实验室开放课题项目(No.APQSKF202404)资助. Supported by the Fujian Public Welfare Project(No.2022R1022001)and the Open Research Project of Fujian Provincial Key Laboratory of Agricultural Product Quality and Safety(No.APQSKF202404). （No.2022R1022001）
