分析化学2026，Vol.54Issue(1)：125-131,7.DOI:10.19756/j.issn.0253-3820.241392
超高效液相色谱-串联质谱法同时检测人血液中的苏沃雷生、达利雷生和莱伯雷生
Simultaneous Detection of Suvorexant,Daridorexant and Lemborexant in Human Blood by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
Suvorexant,daridorexant,and lemborexant,as a class of novel hypnotic agents,have been increasingly reported in cases of drug-facilitated crimes(DFCs),such as sexual assault and poisoning.However,highly selective and sensitive methods for simultaneous detection of these drugs in human blood remain scarce.This study aimed to develop and validate a method based on ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)for simultaneous qualitative and quantitative analysis of these drugs in blood.The sample preparation procedure was systematically investigated and optimized.Chromatographic separation was achieved using UPLC,and MS detection was performed under positive electrospray ionization(ESI+)in multiple reaction monitoring(MRM)mode.The results demonstrated excellent chromatographic performance,with well-resolved peaks and satisfactory separation for all three target analytes.The method exhibited acceptable precision and post-preparative stability.It proved to be highly sensitive,with limits of detection(LOD)of 0.05,0.06 and 0.07 ng/mL,and limits of quantification(LOQ)of 0.17,0.21 and 0.24 ng/mL,for suvorexant,lemborexant,and daridorexant,respectively.The matrix effect,recovery,and accuracy for all analytes met the predefined criteria for bioanalytical method validation.Excellent linearity was observed for the three drugs over a concentration range of 0.25 to 100 ng/mL,with correlation coefficients(r2)greater than 0.998.In conclusion,the developed UPLC-MS/MS method was simple,rapid,sensitive,and reliable,and suitable for routine application in forensic toxicological analysis,showing potential in pharmacokinetic studies of these drugs.关键词
法医毒物学/苏沃雷生/莱伯雷生/达利雷生/超高效液相色谱-串联质谱Key words
Forensic toxicology/Suvorexant/Lemborexant/Daridorexant/Ultra-high performance liquid chromato-graphy-tandem mass spectrometry引用本文复制引用
路林超,王海燕,刘海燕,宋蕊,王婕,武继锋,张广华,栾玉静..超高效液相色谱-串联质谱法同时检测人血液中的苏沃雷生、达利雷生和莱伯雷生[J].分析化学,2026,54(1):125-131,7.基金项目
山东省重点研发计划(重大科技创新工程)项目(No.2021CXGC011305)、中央级公益性科研院所基本科研业务费专项资金项目(No.2022JB015)和北京市现场物证检验工程技术研究中心项目(No.2022CSEEKFKT07)资助. Supported by the Key R&D Program of Shandong Province(Major Science and Technology Innovation Project)(No.2021CXGC011305),the Fundamental Research Funds for Central Public Welfare Research Institutes(No.2022JB015)and the Funded Project by Beijing On-site Physical Evidence Inspection Engineering Technology Research Center(No.2022CSEEKFKT07). （重大科技创新工程）
