福建农业科技2025，Vol.56Issue(11)：1-10,10.DOI:10.13651/j.cnki.fjnykj.2025.11.001
基于表型和ISSR分子标记的福建巴戟天种质资源遗传多样性分析
Genetic Diversity Analysis of Morinda officinalis Germplasm Resources in Fujian Based on Phenotype and ISSR Molecular Markers
摘要
Abstract
In order to evaluate the genetic diversity of Morinda officinalis germplasm resources in Fujian Province,a total of 27 germplasm resources collected from 10 sampling sites across 7 counties in Fujian Province were used as the research objects.Then,the phenotypic traits were systematically recorded and the pollen morphology was observed by using the electron microscopy.The genetic diversity was analyzed by ISSR molecular markers.The results showed that the pollen morphology of M.officinalis in Fujian Province was prolate(the P/E ratio of 1.66 to 1.99),with the polar axis length of 36.89 to 52.93 μm,and the equatorial axis length of 15.14 μm to 33.58 μm.The P/E ratio ranged from from 1.32 to 2.73,and exhibited the highest coefficient of variation(11.36％)among all the pollen traits.The leaf morphology(including elliptic,lanceolate,and ovate forms)and other phenotypic traits such as plant height,leaf number and branch number also displayed substantial variation,with the coefficients of variation ranging from 11.74％to 50.41％.The PCR amplification was performed by using ten ISSR polymorphic primers,generating 64 polymorphic bands with an average polymorphism percentage of 82.48％The results showed that 27 M.officinalis germplasms had high genetic diversity(Nei's gene diversity index H=0.305 2,Shannon's index I=0.457 8).The genetic distance analysis showed that the differentiation between populations was significant(Nei's genetic distance ranged from 0.080 0 to 0.693 1).The genetic relationship between M23 and M24 was the closest(similarity coefficient:0.923 1),and that between M26 and M2 was the farthest(similarity coefficient:0.500 0).The UPGMA clustering analysis divided the germplasm resources into three groups at the genetic distance of 0.654:Group Ⅰ(21 samples)representing the core genetic background,Group Ⅱ(5 samples)sharing common genetic characteristics,and Group Ⅲ(M8)as a unique ecotype.In summary,Morinda officinalis germplasm resources in Fujian exhibited rich genetic diversity,and clear geographical differentiation.It was recommended to give priority to the conservation of populations with high genetic diversity(such as M23/M24 population)and unique ecotypes(M8).The study could provide reference for the conservation,utilization and breeding of germplasm resources of M.officinalis in Fujian.关键词
巴戟天/花粉形态/叶片形态/ISSR分子标记/遗传多样性Key words
Morinda officinalis/Pollen morphology/Leaf shape/ISSR molecular marker/Genetic diversity分类
农业科技引用本文复制引用
朱雁鸣,刘玲玲,牛雨晴,陈宏,李鸾翔,郑梅霞,朱育菁,苏海兰,曾日秋..基于表型和ISSR分子标记的福建巴戟天种质资源遗传多样性分析[J].福建农业科技,2025,56(11):1-10,10.基金项目
福建省科技计划重大专项(2022NZ029017) （2022NZ029017）
漳州市自然科学基金项目(ZZ2025JH46). （ZZ2025JH46）
