福建农业科技2025，Vol.56Issue(11)：11-18,8.DOI:10.13651/j.cnki.fjnykj.2025.11.002
种植竹荪对农田土壤磷有效性及磷循环功能基因丰度的影响
Impact of Dictyophora indusiata Cultivation on Soil Phosphorus Availability and Phosphorus-Cycling Functional Genes Abundance in Farmland
摘要
Abstract
In order to clarify the regulatory mechanisms of Dictyophora indusiata cultivation on the soil phosphorus cycle in paddy fields,the Dictyophora indusiata cultivation plots(JXZS)and the control plots(JXCK)were selected as the experimental subjects.The effects of Dictyophora indusiata cultivation on the soil phosphorus cycle were systematically explored by measuring the soil phosphorus content and the key enzyme activity of phosphorus cycle,combined with the functional genomics analysis.The results showed that:Dictyophora indusiata cultivation increased the soil total phosphorus(TP)content by 54.7%and the available phosphorus(AP)content by 137.5%,concurrently activating the acid phosphatase(ACP)activity by 33.0%.Phytase(PHY)activity exhibited a significant increase,whereas the phosphodiesterase(PDE)activity remained unchanged.(2)The microbial phosphorus metabolic strategy shifted from a"low-phosphorus adaptation"phenotype towards a"high-efficiency utilization"phenotype.The genes such as phosphorus storage(ppk2)and recalcitrant organic P utilization(phn P),alongside the enzyme systems including EC 3.1.4.55(complex organic P degradation)were enriched in the control plots.In contrast,the E3.1.3.8 gene(easily degradable organic phosphorus mineralization)and its corresponding enzyme system were enriched in the cultivation plots.(3)The soil enzyme activities(PHY and PDE)were the key factors driving the microbial functional variation,which were significantly negatively correlated with the core genes such as ppx-gpp A,ppa,while the total phosphorus content exhibited no significant regulatory effect on microbial functionality.(4)The P metabolic network showed the structural characteristics of"loose structure with core node-driven"topology.Within the gene network,only two connector nodes(phn O,E3.1.3.1/pho A/pho B)were identified.E3.1.3.8 and pho N were the core functional genes within the bipartite network,while the majority of microbial genera constituted the peripheral nodes.In conclusion,Dictyophora indusiata cultivation systematically reshaped the phosphorus metabolic functional pattern of soil microorganisms by increasing the soil phosphorus pool capacity and activating the key phosphatases in farmland.This process has led to a shift in microbial strategy from relying on the endogenous phosphorus reserves to prioritizing the utilization of exogenous easily degradable organic phosphorus.The optimization of this metabolic strategy was an important microbiological mechanism for the improvement of soil phosphorus cycling efficiency in Dictyophora indusiata cultivation plots.关键词
竹荪/稻田/土壤磷循环/功能基因/宏基因组学Key words
Dictyophora indusiata/Paddy field/Soil phosphorus cycle/Functional genes/Metagenomics分类
农业科技引用本文复制引用
陈俊华..种植竹荪对农田土壤磷有效性及磷循环功能基因丰度的影响[J].福建农业科技,2025,56(11):11-18,8.基金项目
国家重点研发计划项目子课题(2023YFD1900605-3). （2023YFD1900605-3）
