福建农业科技2025，Vol.56Issue(11)：54-58,5.DOI:10.13651/j.cnki.fjnykj.2025.11.009
大肠杆菌argC基因缺失对各类抗生素的耐受性分析
Tolerance Analysis of Escherichia coli with the Deletion of ArgC Gene to Various Antibiotics
摘要
Abstract
In order to reveal the potential function of the argC gene in Escherichia coli,especially its role in the formation of antibiotic resistance,the tolerance of argC gene knockout strain(ΔargC)was preliminarily evaluated by measuring the survival rate of argC gene knockout strain(ΔargC)under ampicillin treatment,and the growth curve of ΔargC strain was determined to exclude the influence of non-specific physiological effects.On this basis,the tolerance of ΔargC strain to various antibiotics,including amikacin(aminoglycosides),ciprofloxacin(quinolones)and ceftriaxone(β-lactams)was further detected.The results showed that compared with the wild-type strain of E.coli,the survival rate of ΔargC strain was significantly increased under the action of ampicillin,and this phenotype was not caused by growth retardation.The further broad-spectrum antibiotic tests showed that ΔargC strain was resistant to amikacin,ciprofloxacin and other antibiotics,but showed no tolerance to ceftriaxone.In summary,the deletion of argC gene could mediate the development of tolerance to multiple antibiotics in E.coli,which provided a reference for understanding the evolution path of drug resistance and developing the new anti-infection strategies.关键词
大肠杆菌/耐受性/argC基因/抗生素Key words
Escherichia coli/Tolerance/ArgC gene/Antibiotics分类
农业科技引用本文复制引用
陈嘉祺,陈稼骏,姬吉莉,何梅,施源德..大肠杆菌argC基因缺失对各类抗生素的耐受性分析[J].福建农业科技,2025,56(11):54-58,5.基金项目
福建省自然科学基金项目(2025J01402) （2025J01402）
福建省—印尼海洋食品联合研发中心项目(Y1-KF2303). （Y1-KF2303）
