福建农业科技2025，Vol.56Issue(12)：1-9,9.DOI:10.13651/j.cnki.fjnykj.2025.12.001
条纹斑竹鲨躁动死亡病原菌分离鉴定及药敏分析
Identification and Drug Sensitivity Analysis of Pathogenic Bacteria Associated with Agitation and Mortality in Striped Bamboo Shark
摘要
Abstract
In an artificial breeding setting,the striped bamboo sharks exhibited abnormal behavior,agitation,and mortality.In order to identify the causative pathogen of the disease and screen for effective therapeutic agents,a dominant bacterial strain designated CV-P1 was isolated from the spleen tissue of affected sharks.Through the morphological observation,physiological and biochemical characterization,16S rRNA gene sequencing,phylogenetic tree construction,and recurrent infection tests,the strain CV-P1 was comprehensively identified as Vibrio harveyi,with a median lethal dose(LD50)of 1.0×105 CFU·mL-1.The drug susceptibility test indicated that the bacterium was susceptible to 12 antibiotics,including enrofloxacin,neomycin,and thiamphenicol,moderately susceptible to colistin,and resistant to 9 antibiotics,such as florfenicol and tetracycline.Furthermore,the minimum inhibitory concentration(MIC)of each antimicrobial agent against the strain CV-P1 was determined by using the microdilution method.The results indicated that flumequine,sulfafurazole,ceftiofur,ceftazidime,and ofloxacin all had an MIC of 0.25 μg·mL-1;while the MIC values for enrofloxacin,neomycin,thiamphenicol,doxycycline,sulfamonomethoxine,augmentin,and gentamicin were 0.03,2,1,0.125,2,2/1,and 0.5 μg·mL-1,respectively.This study represented the first report of V.harveyi isolated from the diseased striped bamboo sharks,and the results could provide scientific reference for the prevention,control and treatment of bacterial diseases in this species.关键词
条斑纹竹鲨/哈维氏弧菌/分离鉴定/药敏分析Key words
Striped bamboo shark/Vibrio harveyi/Isolation and identification/Drug sensitivity analysis分类
农业科技引用本文复制引用
郭香,叶军,宁岳,巫旗生,吴丽云,葛辉..条纹斑竹鲨躁动死亡病原菌分离鉴定及药敏分析[J].福建农业科技,2025,56(12):1-9,9.基金项目
福建省科技计划省属公益类项目(2025R1013005). （2025R1013005）
