寒旱农业科学2026，Vol.5Issue(1)：64-70,7.DOI:10.3969/j.issn.2097-2172.2026.01.010
三角梅组织培养技术优化研究
Study on Optimization of Tissue Culture Technology in Bougainvillea spectabilis
摘要
Abstract
Rapid propagation through tissue culture is one of the commonly used methods for the large-scale multiplication of elite plant cultivars.To further optimize the in vitro rapid propagation system of Bougainvillea'Angus',semi-lignified nodal segments with axillary buds were used as explants to systematically investigate and refine the optimal conditions for explant disinfection,bud induction,shoot proliferation,rooting,and acclimatization.Results showed that the optimal disinfection treatment was 0.1%HgCl2 for 12 min followed by 0.2%NaClO for 6 min,resulting in a mortality rate of 14.6%and a contamination rate of 25.4%.The optimal proliferation medium was MS supplemented with 1.0 mg/L 6-BA and 0.6 mg/L NAA,which increased the proliferation rate by 34.0%compared to the conventional protocol.The optimal rooting medium was 1/2-strength MS medium containing 0.8 mg/L NAA and 1.5 mg/L IBA.During acclimatization,a substrate mixture of humus soil and vermiculite(1:1,v/v)promoted superior root development and plant establishment.The optimized in vitro propagation system significantly improved the overall seedling production efficiency and reliability of seedling production for Bougainvillea'Angus',overcoming the issues of low repeatability and unstable culture performance in Bougainvillea'Angus'tissue culture,and providing theoretical and practical support for industrialized and large-scale production.关键词
三角梅/安格斯/组织培养/技术体系/优化Key words
Bougainvillea spectabilis/'Angus'/Tissue culture/Technical system/Optimization分类
农业科技引用本文复制引用
席畅,张乐,李壮美,陈乔稳,辛亚龙..三角梅组织培养技术优化研究[J].寒旱农业科学,2026,5(1):64-70,7.基金项目
云南省重大科技计划专项(202302AE090018). （202302AE090018）
