福建农业科技2025，Vol.56Issue(12)：16-25,10.DOI:10.13651/j.cnki.fjnykj.2025.12.003
基于SSR标记与田间鉴定的福建34个水稻主栽品种种子纯度检测对比
Comparison of Seed Purity Detection for 34 Main Cultivated Rice Varieties in Fujian Based on SSR Markers and Field Identification
摘要
Abstract
The purity of rice varieties is a crucial factor affecting the yield and quality.The purity identification is of great significance for ensuring the seed quality and safety,as well as promoting the high-quality development of the agricultural industry.In this study,34 main cultivated rice varieties in Fujian were used as the experimental materials.A total of 48 pairs of SSR primers were employed to conduct the specific primer screening and preliminary identification of seed purity,followed by a comparative analysis with the results of field purity identification.The results showed that through the screening of SSR-specific primers for 34 hybrid rice varieties,40 out of the 48 pairs of SSR primers exhibited the specificity.Among them,the RM432 primer amplified the specific bands in 24 varieties.The selected SSR primers were used to analyze the genotypes of individual plants of the tested varieties.A total of 12 varieties had the seed purity of 96.0%or above,with a qualification rate of 35.3%.When the tolerance gap of 5%was applied,the number of qualified varieties increased to 22,with a qualification rate of 64.7%.Among them,Guangliangyou 676 had the highest purity(100%),while Lvliangyou 9871 had the lowest purity(81.0%).The results of field plot planting identification showed that the purity of all 34 varieties was above 98%,with the qualified rate of 100%.The results showed that there were still some differences between the results of SSR detection and field planting identification,and the molecular marker-based purity identification offered higher precision,which could detect the differences that could not be expressed in morphology.It was recommended that when using the SSR molecular marker technology to carry out the purity identification,the DNA samples from the parental lines of hybrid rice should be used as the control,and the joint identification should be conducted by using more than two pairs of polymorphic primers to enhance the efficiency and accuracy of identification.关键词
SSR标记/水稻/主栽品种/纯度检测/引物Key words
SSR markers/Rice/Main cultivated varieties/Purity detection/Primers分类
农业科技引用本文复制引用
李齐向..基于SSR标记与田间鉴定的福建34个水稻主栽品种种子纯度检测对比[J].福建农业科技,2025,56(12):16-25,10.基金项目
三明市杂交水稻种子产业发展资金(闽政办[2015]95号). （闽政办[2015]95号）
