赣南医科大学学报2026，Vol.46Issue(1)：16-25,10.DOI:10.3969/j.issn.2097-7174.2026.01.003
线粒体自噬调控脊髓损伤的机制及潜在治疗靶点
Mechanisms and potential therapeutic targets of mitophagy regulation in spinal cord injury
摘要
Abstract
Objective:To explore the mechanisms and potential targets of mitophagy in the treatment of spinal cord injury(SCI)to clarify its therapeutic potential,thereby providing novel strategies for improving the prognosis of SCI.Methods:Expression profiles were obtained from the Gene Expression Omnibus(GEO)mouse and spinal cord datasets GSE5296 and GSE47681 to identify differentially expressed genes(DEGs)between SCI and mitophagy-related genes.Analyzed DEGs for pathway enrichment,constructed protein-protein interaction networks,and analyzed immune cell infiltration patterns in SCI.Receiver operating characteristic(ROC)curve analysis was used to evaluate the diagnostic value of DEGs in SCI.Verified the expression levels of key DEGs with reverse transcription-quantitative polymerase chain reaction(q RT-PCR).Analyzed protein levels with western blotting to comprehensively evaluate its biological significance.Results:We identified 55 DEGs associated with SCI and mitophagy,with their enriched signaling pathways closely related to inflammation,apoptosis,and cell survival.Six key genes were identified(Ulk1,Atg14,Map1lc3b,Gabarap,Gabarapl2,and Fundc1).The qRT-PCR analysis results showed that the expression of Atg14,Map1lc3b,and Gabarapl2 was downregulated,while the expression of Gabarap was upregulated.Immune infiltration analysis revealed significant alterations in the abundance of 10 immune cell types after spinal cord injury.Among these,neutrophils showed a strong negative correlation with M0 macrophages(r=-0.56,P<0.05).Meanwhile,Gabarap was significantly positively correlated with M0 macrophages(r=0.58,P<0.05),while Gabarapl2 was significantly negatively correlated with M0 macrophages(r=-0.56,P<0.05).Western blot results indicated increased expression of NF-κB and p-ERK following SCI.Conclusion:By validating the hub genes and identifying immune cell interactions,the potential targets of mitochondrial autophagy in SCI treatment were identified,which pointed out the direction for the subsequent development of effective SCI treatment.关键词
线粒体自噬/脊髓损伤/生物标志物/免疫细胞浸润/分子验证/受试者工作特征曲线Key words
Mitochondrial autophagy/Spinal cord injury/Biomarkers/Immune cell infiltration/Molecular verification/Receiver operating characteristic curve分类
医药卫生引用本文复制引用
吴欣,卢枫,王力峰,张雨,邱嘉欣,韩乐,谢海玉..线粒体自噬调控脊髓损伤的机制及潜在治疗靶点[J].赣南医科大学学报,2026,46(1):16-25,10.基金项目
江西省教育厅科学技术研究项目(编号:GJJ201505) （编号:GJJ201505）
