广州中医药大学学报2026，Vol.43Issue(2)：456-462,7.DOI:10.13359/j.cnki.gzxbtcm.2026.02.025
黄芪多糖通过TLR4/MyD88信号通路调节Th1/Th2失衡改善大鼠过敏性鼻炎
Astragalus Polysaccharides Ameliorate Allergic Rhinitis in Rats by Modulating Th1/Th2 Imbalance via the TLR4/MyD88 Signaling Pathway
摘要
Abstract
Objective To investigate the therapeutic effect and mechanism of Astragalus polysaccharides(APS)on allergic rhinitis(AR)in rats.Methods An AR model was established by intraperitoneal injection of ovalbumin(OVA)and aluminum hydroxide combined with local nasal stimulation with OVA.Successfully modeled rats were randomly divided into a model group,a dexamethasone group,low-dose APS group and high-dose APS group,with 10 rats per group.An additional 10 healthy rats were selected as the normal group.The total score of nasal allergic symptom was assessed before and after treatment.After treatment,histopathological feature in the nasal mucosa were observed by hematoxylin-eosin(HE)staining;serum levels of interferon-gamma(IFN-γ),interleukin-4(IL-4),IFN-γ/IL-4 ratio,and immunoglobulin E(IgE)were detected by enzyme-linked immunosorbent assay(ELISA);and protein expression levels of T-bet,GATA-binding protein 3(GATA-3),and proteins related to the Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)signaling pathway in nasal mucosal tissue were measured by Western Blot.Results Compared with the normal group,the model group exhibited massive exfoliation of nasal mucosal cells,hyperplasia of periglandular tissue in the submucosa,edema,eosinophil infiltration,significantly increased total symptom scores and levels of IL-4,IgE,GATA-3,TLR4,MyD88,and phosphorylated nuclear factor-kappa B(p-NF-κB)(P＜0.05),and significantly decreased levels of IFN-γ,IFN-γ/IL-4 ratio,and T-bet(P＜0.05).Compared with the model group,the dexamethasone group and the low-and high-dose APS groups showed reduced nasal mucosal edema and inflammatory cell infiltration,less epithelial exfoliation,and a more intact nasal mucosal structure,along with significantly decreased total symptom scores and levels of IL-4,IgE,GATA-3,TLR4,MyD88,and p-NF-κB(P＜0.05),and significantly increased levels of IFN-γ,IFN-γ/IL-4 ratio,and T-bet(P＜0.05).Compared with the dexamethasone group,the low-dose and high-dose APS groups showed significantly lower total symptom scores and levels of IL-4,IgE,GATA-3,TLR4,MyD88,and p-NF-κB(P＜0.05),and significantly higher levels of IFN-γ,IFN-γ/IL-4 ratio,and T-bet(P＜0.05).Conclusion Astragalus polysaccharides can effectively alleviate nasal allergic symptoms,inhibit inflammatory responses,and regulate immune function in AR rats.The mechanism is associated with the modulation of TLR4/MyD88 signaling pathway-mediated Th1/Th2 imbalance关键词
黄芪多糖/过敏性鼻炎/TLR4/MyD88信号通路/Th1/Th2失衡/大鼠Key words
Astragalus polysaccharides/allergic rhinitis(AR)/TLR4/MyD88 signaling pathway/Th1/Th2 in balance/rats分类
医药卫生引用本文复制引用
陈进伟,孙继周,蒋源,张仕林..黄芪多糖通过TLR4/MyD88信号通路调节Th1/Th2失衡改善大鼠过敏性鼻炎[J].广州中医药大学学报,2026,43(2):456-462,7.基金项目
陕西省重点研发计划项目(编号:2021SF-379) （编号:2021SF-379）
