家畜生态学报2026，Vol.47Issue(1)：73-78,6.DOI:10.3969/j.issn.1673-1182.2026.01.012
两株鸡源鸭疫里默氏菌的分离鉴定及OmpA基因序列分析
Isolation,Identification and OmpA Gene Sequence Analysis of Two Strains of Riemerella anatipestifer from Chicken
吴蕾 1常海霞 1李玉峰 2韩青海 1刘杰 1韩帅琪 1王东萍 1刘哲 1崔雪志1
作者信息
- 1. 山东中新食品集团有限公司,山东济南 250102
- 2. 山东省农业科学院家禽研究所,山东济南 250023
- 折叠
摘要
Abstract
To identify the two strains of similar pathogenic bacteria isolated from the caseous exudate in the oviduct of broiler breeders and the yolks of their hatching eggs,the two isolates were subjected to Gram staining,serotype identification,drug sensitivity test and OmpA gene analysis.Additionally,patho-genicity tests were conducted on SPF chickens using the isolated bacteria.The results showed that both strains isolated from the oviduct and egg yolk were identified as type 1 Riemerella anatipestifer.Both strains were found to be sensitive to cefotaxime,florfenicol and doxycycline.Phylogenetic analysis of the OmpA gene revealed 100％homology between the two strains,while the homology with reference strains of Riemerella anatipestifer from chickens and ducks ranged from 93.9％～94.0％and 95.8％～99.9％,re-spectively.Injecting bacteria into SPF chickens resulted in gelatinous samples in the joints of chickens.Pathological changes included tubular epithelial cell edema or degeneration and neuronal edema or shrink-age.The experiment showed that broiler chickens can be infected with Riemerella anatipestifer,which may lead to damage to the reproductive system or leg joints following infection.关键词
鸭疫里默氏菌/鸡/分离鉴定/OmpA基因Key words
Riemerella anatipestifer/chicken/isolation and identification/OmpA gene分类
农业科技引用本文复制引用
吴蕾,常海霞,李玉峰,韩青海,刘杰,韩帅琪,王东萍,刘哲,崔雪志..两株鸡源鸭疫里默氏菌的分离鉴定及OmpA基因序列分析[J].家畜生态学报,2026,47(1):73-78,6.
