四川大学学报（医学版）2026，Vol.57Issue(1)：65-72,8.DOI:10.12182/20260160109
代谢相关脂肪性肝病口腔病毒组的特征分析
Analysis of the Characteristics of the Oral Virome in Metabolic Dysfunction-Associated Fatty Liver Disease
摘要
Abstract
Objective To investigate the characteristics of salivary and supragingival plaque viromes in patients with metabolic dysfunction-associated fatty liver disease(MAFLD),and provide new insights for noninvasive oral screening and ecological intervention for MAFLD.Methods This study included 21 MAFLD patients and 20 healthy controls.Saliva and supragingival plaque samples were collected,and metagenomic sequencing was used to analyze the characteristics of the oral virome.Results The α-diversity and β-diversity of the salivary virome did not differ significantly between MAFLD patients and healthy individuals(P>0.05).However,compared with healthy individuals,the α-diversity(Shannon index)and β-diversity(Bray-Curtis distance)of the supragingival plaque virome showed significant differences(P=0.030 3,P=0.001).For species with a relative abundance greater than 0.1%,14 viral species in saliva and 5 in supragingival plaque differed significantly in relative abundance between the two groups(P<0.05),with multiple Streptococcus phages enriched in the saliva of MAFLD patients.LEfSe and random forest analyses identified potential biomarkers in saliva and supragingival plaque.Receiver operating characteristic(ROC)curve analysis showed strong diagnostic performance for these biomarkers in both saliva(area under the curve[AUC]=0.954 8,95%CI:0.889 8-1.000 0)and supragingival plaque(AUC=0.895 2,95%CI:0.777 4-1.000 0).Spearman correlation analysis revealed associations between viral species in saliva or supragingival plaque and various disease indicators(P<0.05).Compared with healthy individuals,MAFLD patients showed higher node counts,significant relationship numbers,and average node degrees in the co-occurrence networks of salivary and supragingival plaque viromes.Conclusion Differences in the species composition and structure of the oral virome between MAFLD patients and healthy individuals suggest that oral viral species could serve as potential biomarkers for diagnosing MAFLD.关键词
脂肪肝/代谢/病毒组/口腔微生态/宏基因组Key words
Fatty liver/metabolism/Virome/Oral microbiota/Metagenome引用本文复制引用
屠叶,牛晨光,黄正蔚..代谢相关脂肪性肝病口腔病毒组的特征分析[J].四川大学学报（医学版）,2026,57(1):65-72,8.基金项目
This study was supported by the National Natural Science Foundation of China(No.82071104,No.82571073),Major and Key Cultivation Projects of Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine(No.JYZP006). 国家自然科学基金(No.82071104、No.82571073)和上海交通大学医学院附属第九人民医院重大重点培育项目(No.JYZP006)资助 （No.82071104,No.82571073）
