四川大学学报（医学版）2026，Vol.57Issue(1)：73-81,9.DOI:10.12182/20260160502
氯己定与保丽净诱导白色念珠菌形成持留菌的机制初探
Chlorhexidine and Polident Induce the Formation of Candida albicans Persistence
摘要
Abstract
Objective To investigate the mechanism by which chlorhexidine and Polident,commonly used denture cleansers,induce the formation of persistence in Candida albicans(C.a.),which is the causative agent of denture stomatitis.Methods C.a.biofilms were formed and treated with 0-40 000 μg/mL chlorhexidine and Polident.Surviving colonies were counted,and dose-and time-dependent curves were plotted.The distribution of persisters was observed via vital staining,and retention was verified by isolating surviving colonies.Transcriptomic analysis was performed on strains treated with chlorhexidine for 2 and 6 hours to explore the mechanisms of persister formation.Metabolic changes in persisters were assessed using the MTT assay.Exogenous glucose was added to validate the role of denture cleansers in regulating persister metabolism.Results After treating C.a.biofilms with chlorhexidine and Polident,the dose-and time-response curves of viable cells both exhibited a biphasic bactericidal pattern,indicating the formation of C.a.persisters.The biofilm structure was disrupted,and persisters were mostly distributed in the middle and upper layers.Transcriptomic analysis showed marked downregulation of the TCA cycle and aerobic respiration,but upregulation of glycolysis.The MTT assay verified that chlorhexidine and Polident suppressed persister metabolism,while the addition of glucose reduced the persister populations;there was a statistically significant difference compared to the control group(P<0.05).Conclusion High concentrations of chlorhexidine and Polident can induce the formation of C.a.persisters,which are mainly distributed in the upper and middle layers of biofilms.The persisters can resuscitate after drug removal,increasing the risk of recurrent infections.C.a.shifts from the tricarboxylic acid cycle and aerobic respiration to glycolysis to maintain a low metabolic level.Exogenous glucose can activate their metabolism and decrease the number of persisters.关键词
义齿性口炎/白色念珠菌/持留菌/氯己定/保丽净Key words
Denture Stomatitis/Candida albicans/Persisters/Chlorhexidine/Polident引用本文复制引用
林祎璠,程磊,周学东,廖玍,朱卓立,任彪..氯己定与保丽净诱导白色念珠菌形成持留菌的机制初探[J].四川大学学报（医学版）,2026,57(1):73-81,9.基金项目
This study was supported by the National Natural Science Foundation of China(No.32470205). 国家自然科学基金面上项目(No.32470205)资助 （No.32470205）
