四川大学学报（医学版）2026，Vol.57Issue(1)：82-89,8.DOI:10.12182/20260160211
乌鲁木齐市学龄前儿童乳牙列期和混合牙列期口腔及肠道微生物组成的差异分析
Differences in Oral and Gut Microbiome Composition Among Preschool Children in Urumqi During the Deciduous and Mixed Dentition Periods
摘要
Abstract
Objective To analyze the oral and gut microbial composition in preschool children during the deciduous and mixed dentition stages,and to investigate the characteristics of microbiota across different dentition stages.Methods Using 16S rRNA gene-based third-generation sequencing,this study analyzed saliva and fecal samples collected from 10 children with deciduous dentition and 10 with mixed dentition.The composition and differences in oral and gut microbiota between the two groups were compared.Results The differences in microbial richness(Chao1 index)and evenness(Shannon index)between the oral cavity and intestine were statistically significant(P=0.001).Firmicutes,Proteobacteria,and Bacteroidetes were the three dominant phyla at both sites.At the genus level,Neisseria and Streptococcus were abundant in the oral cavity,while Bacteroides and Faecalibacterium predominated in the gut.o_Lactobacillus(LDA=5.04,P<0.001)was identified as a marker genus in the oral cavity during the deciduous dentition period,while g_Neisseria(LDA=5.15,P<0.001)served as an oral marker genus in children with mixed dentition.In the gut,marker genera during the deciduous dentition period included o_Bacteroides(LDA=5.17,P<0.001)and f_Lachnospiraceae(LDA=5.16,P<0.001),whereas f_Ruminococcus(LDA=5.09,P<0.001)and g_Faecalibacterium(LDA=5.03,P<0.001)were marker genera during the mixed dentition period.Upregulation of pathways including lactose degradation and nitrate reduction was observed in the oral microbiota,in contrast to the gut microbiota,where enhanced activity of the methanogenesis from acetate pathway was noted.Conclusion The composition of oral and gut microbiota varies among children at different dentition stages.As dentition changes,the structural diversity and metabolic functions of both oral and gut microbiota also change.关键词
乳牙列期/混合牙列期/口腔微生物/肠道微生物/16S rRNAKey words
Deciduous dentition/Mixed dentition/Oral microbiota/Gut microbiota/16S rRNA引用本文复制引用
马婷,连冰洁,吴泽钰,赵今..乌鲁木齐市学龄前儿童乳牙列期和混合牙列期口腔及肠道微生物组成的差异分析[J].四川大学学报（医学版）,2026,57(1):82-89,8.基金项目
This study was supported by the Xinjiang Medical University University-Level Natural Science Youth Research Grant(No.2024XYZR27). 新疆医科大学校级自然科学青年研究项目(No.2024XYZR27)资助 （No.2024XYZR27）
