四川大学学报（医学版）2026，Vol.57Issue(1)：97-106,10.DOI:10.12182/20260160107
西藏凹乳芹乙醇提取物通过HO-1/Nrf2和Myd88/AKT/PI3K信号通路改善大鼠慢性萎缩性胃炎
Ethanol Extract of Vicatia thibetica de Boiss.Improves Chronic Atrophic Gastritis in Rats via the HO-1/Nrf2 and Myd88/AKT/PI3K Signaling Pathways
摘要
Abstract
Objective The ameliorative effects of the ethanol extract of Vicatia thibetica de Boiss.(VTDB)on N-methyl-N'-nitro-N-nitrosoguanidine(MNNG)-induced chronic atrophic gastritis(CAG)in rats were investigated,and its mechanism of action was preliminarily explored.Methods Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)was used to analyze the chemical constituents of VTDB.A rat CAG model was established using MNNG induction combined with irregular feeding.Seventy-two healthy male SD rats were randomly divided into six groups:normal control,model,low-dose VTDB(157 mg/[kg·d]),medium-dose VTDB(314 mg/[kg·d]),high-dose VTDB(628 mg/[kg·d]),and positive control(Vatacoenayme)(216 mg/[kg·d]),with 12 rats in each group.HE staining was used to observe pathological changes in the gastric mucosa of each group.ELISA was performed to measure serum levels of pepsinogen Ⅰ(PGⅠ),pepsinogen Ⅱ(PGⅡ),and inflammatory factors(TNF-α,IL-6,and IL-10).Levels of malondialdehyde(MDA)and superoxide dismutase(SOD)activity in gastric tissues were determined.Western blot was used to measure the expression levels of heme oxygenase-1(HO-1)/nuclear factor erythroid-2-related factor 2(Nrf2)and myeloid differentiation primary response protein 88(MyD88)/protein kinase B(AKT)/phosphatidylinositide 3-kinase(PI3K)signaling pathway proteins in gastric tissues.Results By comparing retention times,mass spectral fragmentation patterns,and matching with the ESI(±)-MS/MS database,a total of 89 chemical constituents-primarily fatty acids,phenolic acids,and coumarins-were identified in VTDB.HE staining indicated that VTDB partially improved gastric mucosal damage in CAG rats.ELISA showed that,compared with the model group,the VTDB-treated groups had increased serum levels of PGⅠ,PGⅡ,and PGR(PGⅠ/PGⅡ)(P<0.05)and decreased levels of gastrin 17(G-17)(P<0.05).In the low-,medium-,and high-dose groups,serum TNF-α levels decreased,and in the low-and high-dose groups,serum IL-6 secretion decreased(P<0.05),while serum IL-10 secretion increased in the high-dose group(P<0.001).In the VTDB medium-and high-dose groups,MDA levels in gastric tissue decreased(P<0.01),and SOD activity increased in the VTDB low-,medium-,and high-dose groups(P<0.05).Western blot results showed that HO-1 levels increased in the high-dose VTDB group and Nrf2 levels increased in the low-,medium-,and high-dose VTDB groups(P<0.001).MyD88 levels decreased in the VTDB low-and high-dose groups,while AKT and PI3K levels decreased in the VTDB medium-and high-dose groups(P<0.05),indicating that VTDB can modulate the expression of HO-1/Nrf2 and MyD88/AKT/PI3K signaling pathway proteins.Conclusion VTDB reduces gastric damage and inflammation in CAG rats by inhibiting lipid peroxidation and inflammatory processes through its effects on the HO-1/Nrf2 and MyD88/AKT/PI3K signaling pathways.关键词
西藏凹乳芹乙醇提取物/化学成分分析/慢性萎缩性胃炎/保护作用Key words
Ethanol extract of Vicatia thibetica de Boiss./Chemical composition analysis/Chronic atrophic gastritis/Protective effect引用本文复制引用
旺杰次仁,兰钧,达娃卓玛,陈敏,杜金洋,叶本贵..西藏凹乳芹乙醇提取物通过HO-1/Nrf2和Myd88/AKT/PI3K信号通路改善大鼠慢性萎缩性胃炎[J].四川大学学报（医学版）,2026,57(1):97-106,10.基金项目
This study was supported by the Key Laboratory Project of Quality Control of Traditional Chinese Medicine(Tibetan Medicine)of National Medical Products Administration(No.XZSYJY-YJKYXM-2023-07)and the Science and Technology Plan Projects of Xizang Autonomous Region(No.XZ202401YD0022,XZ202301YD0014C). 国家药品监督管理局中药(藏药)质量控制重点实验室项目(No.XZSYJY-YJKYXM-2023-07)和西藏自治区科技计划项目(No.XZ202401YD0022、No.XZ202301YD0014C)资助 （Tibetan Medicine）
