核农学报2026，Vol.40Issue(3)：468-478,11.DOI:10.11869/j.issn.1000-8551.2026.03.0468
丹参PERK基因家族的鉴定及表达分析
Identification and Expression Analysis of PERK Gene Family in Salvia miltiorrhiza
摘要
Abstract
Proline-rich extensin-like receptor kinase(PERKs)play critical roles in plant growth,development,and stress responses.To elucidate the functions of the PERK gene family in medicinal plant Salvia miltiorrhiza,we performed a comprehensive genome-wide identification and analysis,such as chromosomal distribution,collinear relationships,phylogenetic relationships,conserved motifs,and cis-acting elements.Furthermore,expression patterns of SmPERK genes across different tissues and under salicylic acid(SA),abscisic acid(ABA),and cucumber mosaic virus(CMV)stress were investigated using transcriptome sequencing and quantitative real-time PCR(qRT-PCR).We identified 20 PERK genes,which were unevenly distributed across six chromosomes.Synteny analysis suggested that gene duplication events were the primary drivers of SmPERK family expansion.Expression profiling revealed distinct tissue-specific patterns:SmPERK12 was highly expressed in flowers,whereas SmPERK11 and SmPERK14 showed preferential expression in roots and stems.Several SmPERK genes responded to SA and ABA treatments,with SmPERK14 exhibiting particularly strong induction by ABA.Upon CMV infection,the expression of most family members was altered,with SmPERK13 and SmPERK14 being significantly upregulated.This study systematically characterizes the SmPERK gene family,clarifying its potential roles in tissue differentiation,hormone signaling,and antiviral defense.The identification of key candidate genes,particularly SmPERK13 and SmPERK14,provides a significant theoretical basis and valuable genetic resources for further understanding the regulatory network governing growth,development,and environmental adaptation mediated by the PERK gene family in S.miltiorrhiza.关键词
丹参/富含脯氨酸伸展蛋白样受体激酶/黄瓜花叶病毒/基因家族表达分析Key words
Salvia miltiorrhiza/PERK/CMV/gene family expression analysis引用本文复制引用
刘硕展,蔡安琪,周景芝,应依铃,叶露晨,俞振明,范慧艳..丹参PERK基因家族的鉴定及表达分析[J].核农学报,2026,40(3):468-478,11.基金项目
国家自然科学基金资助项目(82003890),浙江省基础公益研究计划项目(LTGN24C140003),浙江中医药大学校级科研项目(2025JKZKTS18) （82003890）
