花生学报2026，Vol.55Issue(1)：1-11,11.DOI:10.14001/j.issn.1002-4093.2026.01.001
花生半胱氨酸氧化酶基因AhPCO的克隆与表达分析
Cloning and Expression Analysis of Cysteine Oxidase Gene in Peanut
摘要
Abstract
Research on plant oxygen-sensing mechanisms has demonstrated that cysteine oxidase genes(PCOs)serve as critical oxygen sensors,modulating oxygen-responsive signaling pathways to support the normal growth and development of plant under stress conditions,thereby conferring a significant survival advantage.The coding sequence(CDS)of the peanut AhPCO gene was successfully cloned in this study,which spans 843 bp and encodes a protein consisting of 281 amino acids with a theoret-ical isoelectric point(pI)of 6.06.The protein exhibits hydrophilicity,instability,and lack of signal peptide,and is primarily lo-calized in the nucleus and cytoplasm.Phylogenetic analysis revealed a close evolutionary relationship between the AhPCO protein and AtPCO1 from Arabidopsis thaliana.Analysis of the AhPCO promoter region suggested that the transcription may be in-duced by abscisic acid(ABA)and anaerobic conditions.The qPCR results showed that AhPCO exhibits tissue-specific expres-sion,with the expression levels in different peanut tissues as the following order:seed＞stem＞root＞leaf＞flower.Stress treatment experiments showed that,compared with the control group,AhPCO expression was significantly upregulated after 2 h of high-temperature treatment and 12 h of salt stress.In contrast,AhPCO expression decreased significantly after 15 d of drought stress,and increased sharply while following 1 d of rehydration.Although the expression gradually declined over the subsequent 2 to 4 d,it remained higher than that in the control.The AhPCO gene is significantly regulated by various abiotic stresses,including high temperature,salinity,drought,and rehydration,and exhibits tissue-specific expression patterns.It may play a crucial regulatory role in the stress response and recovery processes of peanuts under environmental stress condi-tions.This study lays a theoretical foundation for elucidating the function of AhPCO gene and provides potential genetic resources for breeding stress-tolerant,high-quality peanut varieties.关键词
花生/半胱氨酸氧化酶(AhPCO)/亚细胞定位/非生物胁迫/基因克隆/表达分析Key words
peanut/plant cysteine oxidases(AhPCO)/subcellular localization/abiotic stress/gene cloning/expression analysis分类
生物科学引用本文复制引用
唐伟,朱振权,冉海霞,石国慧,谭茵研,刘星..花生半胱氨酸氧化酶基因AhPCO的克隆与表达分析[J].花生学报,2026,55(1):1-11,11.基金项目
国家自然科学基金项目(32260441) （32260441）
贵州省科学技术厅基金项目(ZK2021-127) （ZK2021-127）
贵州省大学生创新创业训练项目(ZHCX2023023,ZHCX2022018) （ZHCX2023023,ZHCX2022018）
贵州省高等学校教学内容和课程体系改革项目(2023169) （2023169）
