集美大学学报（自然科学版）2026，Vol.31Issue(1)：34-45,12.DOI:10.19715/j.jmuzr.2026.01.04
二十二碳六烯酸对IgE介导的过敏反应的调节
Regulation of Docosahexaenoic Acid Against IgE-Mediated Allergic Reaction
摘要
Abstract
To explore the regulatory effect of DHA on allergic reaction and the possible mechanism,IgE/BAS-induced RBL-2H3 cells were used as the study object and the release of β-hexosidase was measured to es-tablish the allergy cellular model.Furthermore,histamine and cytokines were determined by ELISA.Calcium ion concentration was determined using the Fluo-3 AM fluorescence probe.The expression levels of cytokines and high affinity receptor FcεRI subunits were determined by quantitative reverse transcription polymerase chain reaction.The expression of FcεRI signal pathway protein was determined by protein immunoblotting method.The results showed that the release amount of β-hexosidase and histamine,as well as the intracellular calcium concentration of sensitized RBL-2H3 cells were significantly reduced upon DHA treatment,suggesting that DHA had an anti-allergic effect.Moreover,the qRT-PCR results showed that DHA treatment significantly reduced the gene expression of FcεRIα subunits without obvious effect on FcεRIβ and FcεRIγ subunit expres-sion.Further Western blot studies showed that DHA significantly inhibited the activity of the PLCγ/JNK and Akt/NF-κB signal pathways in sensitized RBL-2H3 cells,thereby reducing the release of downstream inflam-matory cytokines IL-4 and TNF-α.In summary,DHA inhibits IgE-mediated allergic reactions in RBL-2H3 cells by inhibiting the activity of PLCγ/JNK and Akt/NF-κB signal pathways.关键词
二十二碳六烯酸/过敏反应/细胞因子/不饱和脂肪酸/信号通路Key words
docosahexaenoic acid/allergic response/cytokines/unsaturated fatty acid/signal pathway分类
轻工纺织引用本文复制引用
徐乐,吴梦娜,邵鹏,张丽媛,黄世英,林静瑜,李桂玲..二十二碳六烯酸对IgE介导的过敏反应的调节[J].集美大学学报（自然科学版）,2026,31(1):34-45,12.基金项目
国家自然科学基金项目(31771972) （31771972）
福建省自然科学基金项目(2020J01676) （2020J01676）
水产品深加工技术国家地方联合工程研究中心开放基金项目 （）
