高原地区黄褐斑动物模型不同构建方法的探索
Exploration of Different Methods for Constructing Animal Models of Melasma in Plateau Regions
摘要
Abstract
Objective To investigate suitable methods and efficacy for establishing a melasma animal model under natural high-altitude conditions(Lhasa City,3 650 m)using three approaches:the commonly used progesterone injection plus UV lamp irradiation(Model Ⅰ),and our research group's novel methods of progesterone injection plus natural light irradiation(Model Ⅱ)and natural light irradiation alone(Model Ⅲ).The goal is to provide experimental technical support for subsequent basic research.Methods Twenty female SPF Kunming mice,aged 6-8 weeks and weighing 18-22 g,were selected in the plateau environment.The mice were randomly divided into four groups:progesterone injection plus UV lamp irradiation(Model Ⅰ),progesterone injection plus natural light irradiation(Model Ⅱ),natural light irradiation alone(Model Ⅲ),and a control group(Control),with five mice per group.The apparent status of mice in each group was observed.From days 29 to 40 after modeling,skin physiological parameters of all mice were measured every 2 days,including erythema index(E),melanin(M),skin type angle(ITA),skin lightness(L),redness(a),yellowness(b),and stratum corneum moisture content(CM).After 40 days of modeling,the following biochemical indicators were measured in serum,liver tissue homogenate(LTH),and skin tissue homogenate(STH)from each group:malondialdehyde(MDA),superoxide dismutase(SOD),tyrosinase(TYR),and other biochemical indicators.Correlation analysis was performed between skin physiological parameters and biochemical indicators(based on Model group Ⅰ data).Results Compared with the Control group,the skin irradiation sites in the three Model groups showed dryness and marked hyperpigmentation.Mice in the Model groups also showed blunted responses,wet bedding,increased food intake,and increased urination,while the Control group showed no skin lesions.Skin physiological parameter results indicated that CM values in Model groups Ⅰ,Ⅱ,and Ⅲ were lower than those in the Control group(P<0.05);ITA values in Model groups Ⅰ and Ⅲ were lower than in the Control group(P<0.05);E and a values in all three Model groups were higher than in the Control group(P<0.05);L values in Model group Ⅱ were lower than in the Control group(P<0.05);and b values in Model groups Ⅰ and Ⅲ were higher than in the Control group(P<0.05).Biochemical indicators showed that,compared with the Control group,Model group Ⅰ had increased MDAserum levels(P<0.001),while Model group Ⅲ had elevated MDASTH levels(P<0.05).Additionally,Model group Ⅰ had lower SODLTH and SODSTH activities than Model Ⅱ(P<0.05).Correlation analysis indicated that MDALTH was positively correlated with E value(r=0.72),while MDALTH and MDASTH levels were negatively correlated with L value(r=-0.63,-0.72).Additionally,SODserum activity was positively correlated with ITA(r=0.59).Conclusion Model Ⅰ demonstrated superior modeling efficacy,while Models Ⅱ and Ⅲremained incompletely stabilized.关键词
高原病/黄褐斑/模型,动物Key words
Altitude sickness/Chloasma/Models,animal引用本文复制引用
旦增德琼,方龙伟,尼珍,王瑾,多吉卓玛,央拉,索朗旺堆,德央,巴桑卓玛..高原地区黄褐斑动物模型不同构建方法的探索[J].四川大学学报（医学版）,2026,57(1):243-249,7.基金项目
This study was supported by the Noncommunicable Chronic Diseases-National Science and Technology Major Project(No.2025ZD0551811),Shigatse City 2024 Regional Collaborative Innovation Project(No.QYXTZX-RKZ2024-01-1),Shigatse City 2025 Science and Technology Plan Project(No.RKZ2025ZD-003),2023 Science and Technology Department of Xizang Autonomous Region Project(No.XZ202303ZY0008G),National Natural Science Foundation of China(No.82241023),2024 Project Co-funded by the Science and Technology Department of Xizang Autonomous Region(No.XZ202403ZY0006),Discipline Leading Talents Program of Xizang University(No.0061243/047),and 2024 Annual Project Funded by the Science and Technology Bureau of Lhasa City(No.LSKJ202466). 四大慢病重大专项项目(No.2025ZD0551811)、日喀则市2024年区域科技协同创新专项项目(No.QYXTZX-RKZ2024-01-1)、日喀则市2025年度科技计划项目(No.RKZ2025ZD-003)、2023年度西藏自治区科技厅"揭榜挂帅"项目(No.XZ202303ZY0008G)、国家自然科学基金(No.82241023)、2024年度西藏自治区科技厅联合资助项目(No.XZ202403ZY0006)、西藏大学学科领军人才项目(No.0061243/047)和2024年度拉萨市科技局资助项目(No.LSKJ202466)资助 （No.2025ZD0551811）
