计量学报2026，Vol.47Issue(1)：152-158,7.DOI:10.3969/j.issn.1000-1158.2026.01.18
HIV-1假病毒标准物质的制备及应用研究
Research on the Preparation and Application of HIV-1 Pseudovirus Reference Materials
摘要
Abstract
Based on lentiviral packaging system,the co-transfection technology of multi-plasmid was used to deliver the relevant genes into host cells containing specific nucleic acid sequences,and the human immunodeficiency virus(HIV-1)pseudovirus reference materials were fabricated.The corresponding spike protein was expressed on the outer shell.The standard value of copy number and expanded uncertainly of the reference materials was(300.12±0.54)×104 copies/mL(k=2)by digital polymerase chain reaction(PCR).Taking advantage of the characteristic that the pseudovirus infects by the recognition between the spike protein and the receptor of host cell,the binding could be interfered by adding neutralizing antibodies to inhibit the infection of the pseudovirus.By observing the inhibition degree of neutralizing antibodies at different concentrations on the expression of fluorescence,the Reed-Muench two-formula method was used to calculate the ZPD50 and evaluated the potency of neutralizing antibodies.关键词
生物计量学/人类免疫缺陷病毒/假病毒/数字PCR/中和抗体/标准物质Key words
biometrology/human immunodeficiency virus/pseudovirus/digital PCR/neutralizing antibody/reference materials分类
通用工业技术引用本文复制引用
林婧,范宇宸,雍雪青,张雨晨,黄蓓,王齐鑫..HIV-1假病毒标准物质的制备及应用研究[J].计量学报,2026,47(1):152-158,7.基金项目
江苏省药品监督管理局药品监管科学科研计划(202359) （202359）
