华东理工大学学报（自然科学版）2026，Vol.52Issue(1)：73-82,10.DOI:10.14135/j.cnki.1006-3080.20250326001
光响应多酚基碳点纳米酶超灵敏检测谷胱甘肽
Light-Responsive Polyphenol-Based Carbon Dot Nanozymes for Ultrasensitive Detection of Glutathione
摘要
Abstract
Glutathione(GSH)plays a critical role in cellular redox homeostasis and disease diagnosis,necessitating its efficient detection for biomedical applications.Oxidase(OXD)-like nanozymes offer advantages by eliminating the need for additional oxidants;however,their prolonged exposure to oxygen(O2)often leads to activity decay,which limits practical applications.Here,we report the construction of a light-responsive polyphenol-based carbon dot nanozyme(E-CDs)that leverages the interfacial modulation capabilities of plant polyphenols.E-CDs exhibit exceptional fluorescence stability and OXD-like activity.Under UV-LED irradiation,E-CDs catalyze O2 to generate reactive oxygen species(ROS),while surface polyphenol moieties selectively recognize and capture GSH,facilitating ROS scavenging and enhancing detection sensitivity.This strategy overcomes the intrinsic activity decay of conventional OXD-like nanozymes,achieving an ultra-low detection limit of 5 nmol/L.Our work provides a novel approach for ultrasensitive GSH detection and expands the potential applications of light-responsive nanozymes in biosensing.关键词
光响应/多酚/碳点/纳米酶/谷胱甘肽Key words
light-responsive/polyphenol/carbon dot/nanozyme/glutathione分类
化学化工引用本文复制引用
张亚静,李舒雲,王韬扬,焦云,何云翔,郭俊凌..光响应多酚基碳点纳米酶超灵敏检测谷胱甘肽[J].华东理工大学学报（自然科学版）,2026,52(1):73-82,10.基金项目
国家重点研发计划(2022YFA0912800) （2022YFA0912800）
国家海外高层次人才计划(00308054A1045) （00308054A1045）
国家自然科学基金(22178233 （）
22408241) （）
四川省博士后特别资助(TB2022063) （TB2022063）
