华东理工大学学报（自然科学版）2026，Vol.52Issue(1)：99-108,10.DOI:10.14135/j.cnki.1006-3080.20250416001
大肠杆菌HS27高效生产L-高丝氨酸培养基与补料优化
Strategies for High-Efficiency L-Homoserine Production by Escherichia coli HS27 Optimization of Medium and Feeding
摘要
Abstract
This study established a high-efficiency fermentation process for L-homoserine production using the threonine auxotrophic Escherichia coli strain HS27.The effects of key components in the fermentation medium on product synthesis were systematically investigated.It was demonstrated that the mass ratio of yeast extract powder to corn steep liquor powder,and the mass concentrations of beet molasses and ammonium sulfate significantly impacted L-homoserine production.Further optimization using an orthogonal experimental design determined the optimal medium composition as follows:glucose 15.0 g/L,MgSO4·7H2O 1.5 g/L,yeast extract powder 0.6 g/L,corn steep liquor powder 2.4 g/L,beet molasses 10.0 g/L,L-threonine 0.4 g/L,(NH4)2SO4 3.0 g/L,KH2PO4 1.5 g/L.Under these conditions,the highest shake-flask fermentation titer reached 7.46 g/L,representing a 19%increase compared to the initial conditions.Subsequently,a fed-batch process incorporating a two-stage threonine feeding strategy and betaine feeding was established in a 5 L multi-parameter fermentation bioreactor.By maintaining the oxygen uptake rate(OUR)at(150±5)mmol/(L·h),the L-homoserine titer and synthesis rate were effectively enhanced.Ultimately,an L-homoserine titer of 134.1 g/L and an average synthesis rate of 4.8 g/(L·h)were achieved.This work lays an industrial foundation for the high-efficiency fermentation production of L-homoserine using the engineered Escherichia coli strain HS27.关键词
大肠杆菌/L-高丝氨酸/培养基优化/正交试验设计/L-苏氨酸补料工艺Key words
Escherichia coli/L-homoserine/medium optimization/orthogonal experimental design/L-threonine feeding process分类
生物科学引用本文复制引用
张天宇,杨云龙,杨晨,银肖剑,王志敏,王泽建..大肠杆菌HS27高效生产L-高丝氨酸培养基与补料优化[J].华东理工大学学报（自然科学版）,2026,52(1):99-108,10.基金项目
河北省石家庄市县域特色产业项目(247790652A) （247790652A）
