集美大学学报（自然科学版）2026，Vol.31Issue(1)：16-22,7.DOI:10.19715/j.jmuzr.2026.01.02
性成熟雄性大黄鱼性逆转的诱导
Inducing Sex Reversal in Mature Male Large Yellow Croaker(Larimichthys crocea)
摘要
Abstract
Male large yellow croakers exhibit superiority over females in terms of body size and coloration,and they also tend to have higher survival rates and market prices during later stages of aquaculture.Conse-quently,all-male seedlings are favored by some aquaculture practitioners.Hormone induction represents an ef-fective strategy for achieving monosex breeding in this species.To investigate induced sex reversal,a mixture of estradiol(E2)and a certain proportion of trenbolone acetate(TR)was used as the inducing agent.This mix-ture was formulated into chitosan strips or dissolved in soybean oil to create a sustained-release solution,which was then injected into the abdominal cavity of mature female large yellow croakers.The histological changes of the gonads were observed,and changes in serum sex hormone concentrations,expression levels of male-specific genes,and estrogen receptor genes in the gonads were detected.The results indicated that the induction effect of the E2-TR-soybean oil mixture was superior to that of chitosan-E2-TR strips.When the E2-TR-soybean oil mixture was administered at a dose of 40 μg E2+4 μg TR every 1 kg body weight via intraperitoneal injec-tion for 80 consecutive days,the testes of the experimental group of large yellow croakers degenerated,taking on a morphology similar to ovaries with the formation of numerous oogonia.Notably,serum concentrations of 11-ke-totestosterone significantly decreased,while estradiol concentrations markedly increased(P<0.05).Further-more,the expression of the dmrt1 gene in the gonads was significantly downregulated,while the expression of the esr2 gene was significantly upregulated.The study indicates that inducing sex reversal in mature male large yellow croakers to develop physiological females is feasible.关键词
大黄鱼/性别控制/性成熟雄鱼/性逆转/诱导Key words
large yellow croaker(Larimichthys crocea)/sex control/gonadally mature male fish/sexual re-versal/induction分类
农业科技引用本文复制引用
张家瑞,崔瑜,胡国良,张立宁,任鹏,王志勇..性成熟雄性大黄鱼性逆转的诱导[J].集美大学学报（自然科学版）,2026,31(1):16-22,7.基金项目
宁波市科技计划项目(2021Z002) （2021Z002）
中央引导地方科技发展资金项目(2023ZY1056) （2023ZY1056）
国家海水鱼产业技术体系(CARS-47-G04) （CARS-47-G04）
