昆虫学报2025，Vol.68Issue(12)：1684-1695,12.DOI:10.16380/j.kcxb.2025.12.006
不同性别赤足木蜂成虫肠道细菌多样性分析和功能预测
Diversity analysis and function prediction of the gut bacteria of adult Xylocopa rufipes(Hymenoptera:Apidae)between sexes
摘要
Abstract
[Aim]The gut bacteria of bees play an important role in the host health,physiological characteristics and interaction with the surrounding environment.This study aims to reveal the composition and functional characteristics of the gut bacteria of adult Xylocopa rufipes of different sexes.[Methods]The gut bacterial community composition of adult X.rufipes of different sexes was determined using 16S rRNA high-throughput sequencing technology.Alpha-diversity analysis,beta-diversity analysis and functional prediction of the gut bacteria of adult X.rufipes of different sexes were performed using mothur software,Bray-Curtis distance algorithm and PICRUSt2 software,respectively.[Results]A total of 120 operational taxonomic units(OTUs)of bacteria in the gut of adult X.rufipes were annotated,belonging to 56 species of 36 genera of 20 orders of 10 classes of 7 phyla.Among them,the dominant bacterial phyla were Bacillota,Pseudomonadata and Actinomycetota,with the relative abundance of 49.70％,40.95％and 8.59％,respectively.The detection rates of the dominant bacterial genera Lactobacillus,Gilliamella and Bifidobacterium were over 90％.At the species level,Apilactobacillus micheneri exhibited significantly higher relative abundance in the gut of female adults than in the gut of male adults,while Gilliamella apicola showed no significant difference in relative abundance between sexes.There was no significant difference in alpha-diversity of the gut bacterial community between female and male adults of X.rufipes,but significant difference in bata-diversity of the gut bacterial community between female and male adults of X.rufipes was observed.The relative abundance of genes enriched in metabolic function pathways of the gut bacteria of male adults of X.rufipes was higher than that of female adults.[Conclusion]The gut of adult X.rufipes harbors a stable core bacterial community associated with social bees.Differences in the relative abundance of the dominant gut bacterial community between sexes may be linked to variations in their physiological structures and lifestyles.关键词
赤足木蜂/性别/肠道细菌群落/高通量测序/16S rRNAKey words
Xylocopa rufipes/sex/gut bacteria/high throughput sequencing/16S rRNA分类
生物科学引用本文复制引用
杨乐,张佳卉,王晨昊,陈文阳,韦佳宝,万家,贺春玲..不同性别赤足木蜂成虫肠道细菌多样性分析和功能预测[J].昆虫学报,2025,68(12):1684-1695,12.基金项目
河南省科技攻关项目(162102110101) （162102110101）
国家科技部基础调查专项(2018FY100404) （2018FY100404）
河南南太行山水林田湖草生态保护修复试点工程(济源项目)(JGZJCAI-2019125) （济源项目）
