临床神经外科杂志2026，Vol.23Issue(1)：30-35,6.DOI:10.3969/j.issn.1672-7770.2026.01.006
胶质母细胞瘤类器官构建方式的改良及其在电场治疗研究中的应用
Improved method for obtaining glioblastoma organoid and its application in research of tumor-treating field
摘要
Abstract
Objective To improve the method of establishing glioblastoma organoids(GBO)and explore the feasibility of evaluating the effect of new treatments.Methods The postoperative pathological tissues of glioblastoma(GBM)diagnosed as WHO grade Ⅳ were collected,and the tumor organoids and primary cells from different patients were established and compared with the parental tumors,and the GBO were used to evaluate the effect of tumor-treating field treatment in vitro.Results Fifteen of the eighteen GBM tissues were successfully established and retained the structural characteristics of the parental tumors,which could be cultured for a long time and used for experiments.Primary GBM cells were obtained during the process,and expressed CD133 in immunofluorescence staining.In vitro experiments confirmed that TTF treatment could inhibit the growth of GBO.Conclusions This study improved a low-cost method to rapidly establish GBO to evaluate the effect of tumor-treating field treatment.关键词
胶质母细胞瘤/类器官/原代细胞/电场治疗Key words
glioblastoma/organoid/primary cell/tumor-treating field分类
医药卫生引用本文复制引用
王思源,师忠港,戴大伟,叶振鸿,缪亦锋,邱永明..胶质母细胞瘤类器官构建方式的改良及其在电场治疗研究中的应用[J].临床神经外科杂志,2026,23(1):30-35,6.基金项目
国家自然科学基金面上项目(82272728) （82272728）
