昆虫学报2025，Vol.68Issue(12)：1725-1735,11.DOI:10.16380/j.kcxb.2025.12.010
柳蓝叶甲滞育前期和产卵前期雌成虫能量贮备模式差异
Differences in the energy storage patterns between pre-diapause and pre-oviposition adult females of Plagiodera versicolora(Coleoptera:Chrysomelidae)
摘要
Abstract
[Aim]For adult diapause insects,the development patterns of ovaries and flight muscles during the pre-diapause period and pre-oviposition period exist obvious differences.Insects require substantial energy inputs during both diapause and reproduction stages,but it is unclear whether the energy storage patterns differ between the pre-diapause period and the pre-oviposition period.The willow leaf beetle Plagiodera versicolora overwinters as adults.The purpose of this study is to clarify the changes in the energy storage patterns in female adults of P.versicolora during the pre-diapause period and pre-oviposition period.[Methods]The female adults of P.versicolora during the pre-diapause period and pre-oviposition period were obtained by keeping P.versicolora under the conditions of the temperature 22 ℃ and a long-day photoperiod 16L∶8D or a short-day photoperiod 12L∶12D.The differences in the body weight and head width of female adults of P.versicolora as well as the weight and morphological development of their ovaries and flight muscles were systematically compared during the pre-diapause period and pre-oviposition period.Meanwhile,the differences in the contents of lipid,glycogen and soluble protein in the entire bodies,flight muscles,ovaries and fat bodies of female adults were compared during the pre-diapause period and pre-oviposition period.[Results]The body weight and head width of the female adults of P.versicolora exhibited no significant difference between the pre-diapause period and the pre-oviposition period,and the lipid contents in the entire body of female adults were similar between the pre-diapause period and the pre-oviposition period,but at 4,6 and 8 d after adult emergence,the glycogen contents in the entire body of female adults during the pre-oviposition period were significantly higher than those during the pre-diapause period,being 1.33-,1.35-and 1.30-fold,respectively,as high as those during the pre-diapause period.The flight muscles and ovaries of female adults were also more developed during the pre-oviposition period than during the pre-diapause period.The lipid,soluble protein and glycogen contents in the flight muscles of pre-oviposition female adults were 1.30-2.52-fold as high as those of pre-diapause female adults at 4,6 and 8 d after adult emergence.Similarly,the lipid and soluble protein contents in the ovaries of female adults were significantly higher during the pre-oviposition period than during the pre-diapause period.By contrast,the lipid,soluble protein and glycogen contents in the fat body of female adults were significantly higher during the pre-diapause period than during the pre-oviposition period.The lipid,soluble protein and glycogen contents in the fat body of pre-diapause female adults were 1.25-1.57-fold as high as those of pre-oviposition females at 6 and 8 d after adult emergence.[Conclusion]The energy storage patterns of the female adults of P.versicolora differ obviously during the pre-diapause period and pre-oviposition period.The female adults during the pre-oviposition period utilize stored energy to facilitate development of the flight muscles and ovaries,thereby enhancing their flight and reproductive capabilities.By contrast,the female adults during the pre-diapause period utilize a greater proportion of energy stored in the fat body to support their survival through the winter season.The findings obtained in this study provide insights into the mechanisms that govern the regulation of development and the utilization of energy reserves during diapause and reproduction.关键词
柳蓝叶甲/滞育/生殖/能量贮备/脂肪/可溶性蛋白质Key words
Plagiodera versicolora/diapause/reproduction/energy reserve/lipid/soluble protein分类
生物科学引用本文复制引用
郑俊超,徐铭暄,赵吕权,李同浦..柳蓝叶甲滞育前期和产卵前期雌成虫能量贮备模式差异[J].昆虫学报,2025,68(12):1725-1735,11.基金项目
国家自然科学基金项目(32171803) （32171803）
江苏省高等学校基础科学研究重大项目(22KJA220002) （22KJA220002）
