林业科学2026，Vol.62Issue(2)：154-159,6.DOI:10.11707/j.1001-7488.LYKX20250074
亚洲象粪便病毒宏基因组学研究:病毒群落组成及保护意义
Study on the Fecal Virus Metagenome of Asian Elephant:Viral Community Composition and Conservation Implications
摘要
Abstract
[Objective]This study aims to investigate the fecal virus metagenome of captive and wild Asian elephants in Yunnan,so as to provide a theoretical foundation for the conservation of this endangered species.[Method]Fresh fecal samples from captive breastfed young elephants,captive adult elephants,and wild adult elephants were collected.The metagenomic sequencing and virus metagenomics analysis methods were used to comprehensively understand the landscape structure of the Asian elephant fecal DNA virus community.[Result]The results revealed that Myoviridae emerged as the most prevalent virus family in the fecal matter of calves,whereas Siphoviridae dominated in the adult elephants.Wild adults exhibited clearly higher Podoviridae abundance than captive adults.At the species level,Synechococcus phage S-CRM01 and Erwinia phage Ea35-70 were dominant in calves,whereas uncultured Mediterranean phage uvMED was most prevalent in adults.Wild adults showed notably higher abundance of EBPR podovirus 3 than captive adults.Furthermore,this study uncovered the presence of herpesvirus in the fecal samples of the calves,having the possible association of this virus family with EEHV.[Conclusion]There are significant differences in the composition of fecal viral communities between captive and wild elephants,as well as between calves and adults.This work sheds light on the possible risk of EEHV infection among Asian elephant populations in Yunnan,and suggests the need for proactive conservation strategies and continued surveillance efforts.关键词
亚洲象/粪便病毒群落/病毒宏基因组/疱疹病毒Key words
Asian elephant/fecal virus composition/viral metagenome/herpesvirus分类
农业科技引用本文复制引用
张呈波,保明伟,黄遵锡,陈军民,韩楠玉,许波,高以任,陈紫娴,王佳雯,沈庆仲,王斌..亚洲象粪便病毒宏基因组学研究:病毒群落组成及保护意义[J].林业科学,2026,62(2):154-159,6.基金项目
国家自然科学基金项目(32260015) （32260015）
云南省科技计划项目基础研究专项面上项目(202401AT070131,202501AT070014) （202401AT070131,202501AT070014）
云南省教育厅科学研究基金(2023J0202) （2023J0202）
云南省"兴滇英才支持计划"青年人才科研支持项目(XDYC-QNRC-2023-0507). （XDYC-QNRC-2023-0507）
