农药学学报2026，Vol.28Issue(1)：181-189,9.DOI:10.16801/j.issn.1008-7303.2026.0007
四氯虫酰胺对斑马鱼的急性毒性及肝脏功能的影响
Acute toxicity and liver function effects of tetrachlorantraniliprole on zebrafish
摘要
Abstract
Tetrachlorantraniliprole is a kind of bisamide insecticide developed independently in China,known for its high efficiency and prolonged duration of action.In view of the potential adversely effect of diamide insecticides on aquatic organisms,such as chlorantraniliprole,safety assessment of tetrachlorantraniliprole is particularly important,especially its impacts on aquatic life.In this study,zebrafish(Danio rerio)were selected as the model organism,and the acute toxicity of chlorantraniliprole and its effect on liver function of adult zebrafish were observed using the semi-static exposure method.The acute toxicity test showed that the 96 h-LC50 value of tetrachlorantraniliprole to zebrafish was 9.8 mg/L,belonging to the toxic grade.In terms of zebrafish growth,exposure to tetrachlorantraniliprole for 7 and 14 days did not significantly change the body weight,body length,and body mass index(BMI)of zebrafish.However,the hepatic somatic index(HSI)decreased significantly after 14 days.Exposure to 0.1 mg/L tetrachlorantraniliprole for 7 days significantly increases glutathione peroxidase(GPx)activity,while exposures to 0.01 and 1 mg/L for 14 days significantly increase glutathione(GSH)content.Histopathological analysis showed that exposure to tetrachlorantraniliprole caused hepatic dysfunction in zebrafish,with the severity of hepatocellular lesions increasing in a concentration-dependent manner.The detection of the mRNA expression levels of apoptosis-related genes showed that the mRNA expression levels of Caspase3,Caspase9,and Apaf1 were significantly increased after exposure to 0.01,0.1,and 1 mg/L tetrachlorantraniliprole for 7 days,suggesting tetrachlorantraniliprole affected the apoptosis of zebrafish liver cells.In conclusion,tetrachlorantraniliprole has certain acute toxicity and induces liver oxidative damage in zebrafish,and its potential risks to the environment and non-target organisms warrant serious attention.These findings provide a theoretical basis for ecological risk assessment of tetrachlorantraniliprole pesticide.关键词
四氯虫酰胺/斑马鱼/急性毒性/肝脏功能Key words
tetrachlorantraniliprole/zebrafish/acute toxicity/liver function分类
农业科技引用本文复制引用
刘文军,卢倩芸,陈杨,王霞云,徐王瑾,孟志远,陈小军..四氯虫酰胺对斑马鱼的急性毒性及肝脏功能的影响[J].农药学学报,2026,28(1):181-189,9.基金项目
国家自然科学基金(No.42407375) （No.42407375）
江苏省重点研发计划项目(No.BE 2023327)扬州市重点研发计划项目(No.YZ 2023038) （No.BE 2023327）
绿色农药全国重点实验室(华南农业大学)开放基金(No.gplscau202417). Supported by National Natural Science Foundation of China(No.42407375) （华南农业大学）
Key Research and Development Program of Jiangsu Province(No.BE 2023327) （No.BE 2023327）
Key Research and Development Program of Yangzhou City(No.YZ 2023038) （No.YZ 2023038）
Open Fund of State Key Laboratory of Green Pesticide,South China Agricultural University(No.gplscau202417). （No.gplscau202417）
