华西口腔医学杂志2026，Vol.44Issue(1)：94-105,12.DOI:10.7518/hxkq.2025.2025050
香芹酚通过磷脂酰肌醇3-激酶途径诱导牙周炎牙槽骨修复的机制研究
Mechanism underlying carvacrol-induced alveolar bone repair via the phosphatidylinositol 3-kinase pathway in periodontal disease
摘要
Abstract
Objective This study aims to explore the mechanism of carvacrol(CV)in regulating alveolar bone repair in periodontal disease.Methods A rat model of periodontal disease(P group)was created by ligating the first molar and injecting lipopolysaccharide(LPS).A control model(C group)was also created.The treatment models received low(L group),medium(M group),and high(H group)doses of CV hydrogel.Hematoxylin-eosin(HE)staining was used to observe the pathological changes in periodontal tissues.Immunohistochemical staining was employed to analyze the ex-pression of collagen typeⅠ(COL1)and Runt-related transcription factor 2(Runx2).In vitro,the rat osteoblast cells were divided into C,P,L,M,H,CV and CV+LY294002(CV+LY)groups.Western blot analysis detected the phosphatidylino-sitol 3-kinase(PI3K)/protein kinase B(AKT)/glycogen synthase kinase 3 beta(GSK-3β)pathway-related and os-teoblastic proteins.Quantitative reverse-transcription po-lymerase chain reaction was used to measure the expres-sion of inflammatory factors and osteoblastic proteins.The alkaline phosphatase(ALP)colorimetric kit and alizarin red S staining kit were utilized to assess osteogenic ability.Immunofluorescence(IF)was used to detect COL1 expression in osteoblasts.Transmission electron microscopy was ap-plied to detect cell apoptosis.Results CV hydrogel alleviated periodontal symptoms,upregulated PI3K/AKT/GSK-3β pathway-related and osteoblastic proteins,and increased the expression of ALP and the number of calcified nodules.However,it decreased cell apoptosis and inflammatory factors.LY294002 inhibited the PI3K/AKT pathway and de-creased osteoblastic protein expression,ALP coloration,and calcified nodule quantity.Conclusion CV hydrogel pro-motes the proliferation and differentiation of alveolar bone osteoblasts by activating the PI3K pathway and inhibiting in-flammation-induced bone resorption.This study emphasizes the potential of CV for the treatment of periodontal diseases.关键词
香芹酚/牙周炎/成骨细胞/磷脂酰肌醇3-激酶/脂多糖Key words
carvacrol/periodontal disease/osteoblasts/phosphatidylinositol 3-kinase/lipopolysaccharide分类
医药卫生引用本文复制引用
滕念,李兆勋,高甜甜,项艳蕊,周露露,高翔..香芹酚通过磷脂酰肌醇3-激酶途径诱导牙周炎牙槽骨修复的机制研究[J].华西口腔医学杂志,2026,44(1):94-105,12.基金项目
"Excellent Youth"Project in Heilongjiang Province in 2023(YQ2023C016) 2023年黑龙江省"优秀青年"项目(YQ2023C016) （YQ2023C016）
