南京医科大学学报（自然科学版）2026，Vol.46Issue(2)：202-212,11.DOI:10.7655/NYDXBNSN250503
下调Dixdc1表达抑制创伤性脑损伤后星形胶质细胞极化
Downregulation of Dixdc1 expression suppresses astrocyte polarization following traumatic brain injury
摘要
Abstract
Objective:To investigate the effects of DIX domain-containing protein 1(Dixdc1)on the proliferation,migration,and polarization of astrocytes following traumatic brain injury(TBI)in mice.Methods:A mouse TBI model was established using the controlled cortical impact(CCI)device.Western blot was used to examine the expression of Dixdc1,glial fibrillary acidic protein(GFAP),the neurotoxic astrocyte marker complement 3(C3),and the neuroprotective astrocyte marker S100 calcium-binding protein A10(S100A10)before and after injury.Immunohistochemical staining was performed to observe the expression and distribution of Dixdc1 and GFAP,and immunofluorescence staining was used to observe the colocalization of Dixdc1 with GFAP.Short hairpin RNA(shRNA)was used to knock down Dixdc1 levels in mouse astrocytes C8-D1A.Lipopolysaccharide(LPS)stimulation was then applied to mimic the activated state of astrocytes in vitro.Western blot was performed to detect expression changes of Dixdc1,GFAP,C3,S100A10,as well as cell proliferation and migration-related proteins.Flow cytometry was performed to analyze the cell cycle,accompanied by Western blot detection of S-phase-related proteins.RT-PCR was used to detect the expression of astrocyte polarization markers.Cellular immunofluorescence was employed to detect the fluorescence intensity of C3 and S100A10.Additionally,Western blot was performed to assess the phosphorylation levels of signal transducer and activator of transcription 3(STAT3).Results:Compared with the Sham group,the protein expression levels of Dixdc1 and GFAP were upregulated in the cerebral cortex of TBI mice.Dixdc1 was expressed in astrocytes within the peri-lesional cortical regions.Following LPS stimulation,the protein expression levels of both Dixdc1 and GFAP were upregulated in C8-D1A cells.Scratch assay and EdU assay demonstrated that knockdown of Dixdc1 suppressed LPS-induced astrocyte proliferation and migration,downregulated the expression of associated proteins,reduced the proportion of cells in the S phase of the cell cycle,and decreased CyclinA and CDK2 protein levels.C3 showed elevated expression levels during the acute phase but declined thereafter,whereas S100A10 demonstrated an inverse temporal pattern.Dixdc1 knockdown may inhibit LPS-induced A1-type astrocyte polarization by reducing STAT3 phosphorylation levels,which concurrently downregulated C3 protein expression and upregulated S100A10 protein expression.Conclusion:Following TBI,Dixdc1 protein expression was upregulated in astrocytes.Knockdown of Dixdc1 significantly suppressed LPS-induced proliferation,migration,cell cycle and STAT3 phosphorylation of A1-type astrocytes.关键词
创伤性脑损伤/星形胶质细胞/增殖/迁移/极化/Dixdc1Key words
traumatic brain injury/astrocytes/proliferation/migration/polarization/Dixdc1分类
医药卫生引用本文复制引用
汤莉巧,段程伟,陈伟观,郭宇,孙王妍,宰添羽,刘梦琦,张冬梅,卢红建..下调Dixdc1表达抑制创伤性脑损伤后星形胶质细胞极化[J].南京医科大学学报（自然科学版）,2026,46(2):202-212,11.基金项目
国家重点研发计划(2022YFC2405605) （2022YFC2405605）
国家自然科学基金(32101027) （32101027）
江苏省自然科学基金(BK20221274) （BK20221274）
南通市科技计划项目(JC2023114) （JC2023114）
江苏省医学重点学科(实验室)建设单位(JSDW202249) （实验室）
