山东科学2026，Vol.39Issue(1)：11-20,10.DOI:10.3976/j.issn.1002-4026.2025048
基于"指纹图谱-模式识别-血清移行成分"整合策略筛选清消五味汤质量差异标志物
Screening quality-difference markers of Qingxiao Wuwei Decoction using an integrated"fingerprint-pattern recognition-serum migration components"strategy
摘要
Abstract
By establishing high-performance liquid chromatography(HPLC)fingerprints of Qingxiao Wuwei Decoction(QXWWD)and integrating chemical pattern recognition with in vivo and in vitro component identification methods,this study aimed to screen differential compounds and provide reference data for consistency evaluation and the development of compound preparations.The fingerprints of 18 batches of QXWWD were established evaluated for similarity.Bioinformatics methods were used to analyze potential quality markers.HPLC-Q-Exactive-MS was used to identify in vitro chemical components and serummigration components of QXWWD.Based on the integrated"fingerprint-pattern recognition-serum-migrant components"strategy,quality-difference markers were screened.A total of 28 peaks were identified in the fingerprints of QXWWD,among which 8 quality-difference markers,such as chrysophanol,were selected.In total,85 in vitro chemical components and 55 serummigration components of QXWWD were identified.By finding the intersection of the fingerprint-identified components and those identified in vivo and in vitro using a Venn diagram,we identified chrysophanol as the core quality-difference marker.This study established a simple and reproducible analytical method that can effectively evaluate the quality stability and batch consistency of QXWWD.This method provides reliable data to support process optimization,quality-standard development,and the subsequent development of this prescription.关键词
清消五味汤/指纹图谱/模式识别/血清移行成分/质量标志物Key words
Qingxiao Wuwei decoction/fingerprint/pattern recognition/serummigration components/quality difference markers分类
医药卫生引用本文复制引用
施凯桃,李君,张谦,马宇衡..基于"指纹图谱-模式识别-血清移行成分"整合策略筛选清消五味汤质量差异标志物[J].山东科学,2026,39(1):11-20,10.基金项目
国家自然科学基金项目(82360801) （82360801）
内蒙古自然科学基金(2025LHMS08061) （2025LHMS08061）
内蒙古医科大学面上项目(YKD2023MS085) （YKD2023MS085）
内蒙古公立医院联合基金(2024GLLH0248) （2024GLLH0248）
内蒙古高等教育科学研究项目(NJZY21591) （NJZY21591）
内蒙古医科大学英才培育项目(YCPY2025024,YCPY2025028) （YCPY2025024,YCPY2025028）
内蒙古医科大学大学生创新创业训练计划项目(101322025034) （101322025034）
