山东科学2026，Vol.39Issue(1)：43-52,10.DOI:10.3976/j.issn.1002-4026.2025085
基于斑马鱼模型对Betukladin降血脂活性与机制分析
Analysis of the lipid-lowering activity and mechanism of Betukladin based on a zebrafish model
摘要
Abstract
Betukladin is a dietary supplement composed of reindeer lichen powder and birch bark extract,possessing various pharmacological activities such as blood glucose regulation,lipid lowering,anti-inflammatory effects,and anticoagulation.This study investigates the lipid-lowering activity and underlying mechanism of Betukladin,aiming to validate its efficacy and provide a scientific basis for developing safe and economical natural lipid-lowering drugs.Using a zebrafish hyperlipidemia model,we found that Betukladin significantly improved the elevated Oil Red O staining integrated optical density values,triglyceride levels,and total cholesterol levels induced by high-fat modeling,indicating its lipid-lowering activity.Network pharmacology analysis suggested that Betukladin may exert its lipid-lowering effects through multiple coordinated pathways,including lipid and atherosclerosis pathways and the PPAR signaling pathway.RT-qPCR results showed that it can regulate lipid metabolism-related genes(apoA1,LDLR,and ANGPTL3),fatty acid synthesis and oxidation genes(SREBF1,FASN,PPARα,and cpt1a,),and cholesterol metabolism-related genes(CYP7A1,HMGCR,ABCG5,and ABCG8).关键词
高脂血症/Betukladin/驯鹿地衣/白桦树皮/降血脂/斑马鱼模型Key words
hyperlipidemia/Betukladin/reindeer lichen/birch bark/lipid lowering/zebrafish model分类
医药卫生引用本文复制引用
杜兴硕,展文,高松英,孙长高,徐明贤,张思晨,李洪亮,夏青,何秋霞..基于斑马鱼模型对Betukladin降血脂活性与机制分析[J].山东科学,2026,39(1):43-52,10.基金项目
山东省自然科学基金创新发展联合基金(ZR202306200045) （ZR202306200045）
中央引导地方科技发展资金项目(YDZX2022169) （YDZX2022169）
