摘要
Abstract
Objective To compare the positive rates of fluorescence quantitative PCR and liq-uid culture methods in detecting Ureaplasma urealyticum(Uu)and to conduct drug susceptibility analysis on specimens with isolated Uu positivity,thereby providing evidence for the clinical diag-nosis and treatment of Uu infections.Methods A retrospective analysis was performed on the re-sults of Uu detection by PCR and liquid culture methods in 368 cases at Chongqing Traditional Chinese Medicine Hospital from January 2022 to December 2024.The positive rates of Uu detec-tion by the two methods were compared.The data from samples positive for Uu by both methods were analyzed,and drug susceptibility analysis was conducted on specimens with isolated Uu posi-tivity.Results There was a significant difference in the positive rates of Uu detection between PCR and liquid culture methods(P=0.004).A total of 71 patients were positive for Uu by both methods,yielding a positive rate of 19.29%,including 32 females(35.56%)and 39 males(14.03%).The proportion of females was significantly higher than that of males(x2=20.23,P＜0.001).Additionally,98 cases were positive only by liquid culture method,representing a positive rate of 26.63%,while 80 cases were positive only by PCR method,with a positive rate of 21.74%.The consistency of detection results between the two methods was moderate(Kappa=0.734).The majority of patients who tested positive for Uu by both methods were concentrated in the 20～39 age group.Among 58 cases with isolated Uu positivity,the susceptibility rates to mi-nocycline and josamycin were both 100%,and the susceptibility rates to doxycycline and clar-ithromycin were all above 90%.Conclusions There are differences in the positive rates of Uu de-tection between fluorescence quantitative PCR and liquid culture methods.Combined detection u-sing both methods should be considered when necessary to enhance diagnostic accuracy.In clini-cal practice,minocycline,josamycin,doxycycline,and clarithromycin,which demonstrate high susceptibility,can be recommended as first-line treatments for Uu-positive patients.关键词
解脲脲原体/荧光定量PCR/液体培养法Key words
Ureaplasma urealyticum/fluorescence quantitative PCR/liquid culture method
