水产学报2026，Vol.50Issue(2)：170-183,14.DOI:10.11964/jfc.20250515008
富硒粪肠球菌对大口黑鲈生长性能、肠道菌群稳态与抗病能力的影响
Effects of selenium-enriched Enterococcus faecalis on growth performance,gut microbiota homeostasis,and disease resistance in largemouth bass(Micropterus salmoides)
摘要
Abstract
To investigate the effects of selenium-enriched Enterococcus faecalis(EFSe)on growth performance,antioxidant capacity and disease resistance in largemouth bass(Micropterus salmoides).E.faecalis MSEF22 was isolated from intestinal tract of healthy largemouth bass and enriched with sodium selenite to obtain EFSe.Three groups were designed:control(basal diet),EF(basal diet+1×107 CFU/g E.faecalis MSEF22),and EFSe(basal diet+1×107 CFU/g EFSe,0.76 mg Se/kg).After 42-day feeding,growth indices,hepatic antioxidant enzymes and intestinal microbiota were analyzed.Disease resistance was eval-uated by 14-day challenge with Nocardia seriolae.EFSe group showed significantly higher WGR,hepatic SOD activity and GSH content than control(P<0.05),while serum AST activity decreased markedly.Compared with EF group,EFSe signific-antly increased gut microbial diversity and abundance of beneficial bacteria(e.g.Lactobacillus spp.).Post-challenge survival rate in EFSe group(52.22%)was higher than EF(50.00%)and control groups(43.33%),with reduced hepatic nodules and downregulated mRNA expression of pro-inflammatory cytokines IL-1β(-76%)and TNF-α(-23%)(P<0.05).Dietary supple-mentation with EFSe enhances growth performance,antioxidant capacity,and disease resistance in largemouth bass,demon-strating significant potential for eco-efficient and sustainable aquaculture.关键词
大口黑鲈/粪肠球菌/富硒/肉芽肿/(鰤)诺卡氏菌Key words
Micropterus salmoides/Enterococcus faecalis/selenium-enriched/sarcoidosis/Nocardia seriolae分类
农业科技引用本文复制引用
张韵,余鹏辉,苏磊,苏建国,高宇,袁改玲..富硒粪肠球菌对大口黑鲈生长性能、肠道菌群稳态与抗病能力的影响[J].水产学报,2026,50(2):170-183,14.基金项目
国家重点研发计划(2024YFE0112200) （2024YFE0112200）
2022年云南省"兴滇英才支持计划"青年人才项目 National Key Research and Development Program of China(2024YFE0112200) （2024YFE0112200）
Young Talent Project of the"Xing Dian Talent Support Program"of Yunnan Province in 2022 （）
