南京师大学报（自然科学版）2026，Vol.49Issue(1)：135-143,9.DOI:10.3969/j.issn.1001-4616.2026.01.014
聚乙烯颗粒对三氯生在蚯蚓体内富集的影响及其复合污染的毒性效应
Effect of Polyethlene Microplastics on the Bioaccumulation and Toxicity of Triclosan in Earthworms
摘要
Abstract
Microplastics(MPs)can absorb various pollutants and alter the biological accumulation,distribution,and toxic effects of other pollutants.Nevertheless,few studies have focused on these combined effects on soil organisms.In this paper,Eisenia foetida was used to evaluate the effect of polyethylene(PE)particles of varying sizes(mesh numbers 30 and 100)and concentrations(0.2％and 2％)on the triclosan(TCS)accumulation and distribution in earthworms at individual,sub-organism,subcellular,and tissue levels.Furthermore,the combined toxic effects on earthworms were investigated.The results revealed that PE particles reduced the accumulation of TCS in earthworms—i.e.,the higher the PE concentration and the greater the amount of PE in the soil,the lower the accumulation of TCS in earthworms.The TCS distribution at the sub-organism level was clitellum>pre-clitellum>post-clitellum,while it was extracellular>intracellular at the subcellular level and intestinal>epidermis at the tissue level.PE particles significantly decreased the TCS distribution in the pre-clitellum,extracellular,and intestinal regions.Superoxide dismutase(SOD),malondialdehyde(MDA),and 8-hydroxydeoxyguanosine monophosphate(8-OHdG)production were induced to resist antioxidant stress caused by the increase in the PE amount and TCS exposure in earthworms.K+Na+-ATP and Ca2+-ATP enzymes exhibited comparable characteristics,thus maintaining ion balance in and out of cells.The induction effect of the TCS exposure group was stronger than that of the PE particles groups.Overall,compared with TCS treatments alone,the combined exposure to PE(ingested and uningested PE particles)and TCS did not enhance oxidative stress-related enzymes.However,the ingested PE particles induced the 8-OHdG index and inhibited the AKP,K+Na+-ATP,and Ca2+-ATP enzyme indices,suggesting that PE increased the effect of DNA damage induced by TCS on earthworms and attenuated the interference of TCS with ion balance in and out of cells in earthworms.These results provide a scientific basis for understanding the impact of microplastics on the ecological risk posed by soil organic pollutants.关键词
微塑料/三氯生/蚯蚓/富集分布/酶活性Key words
microplastics/triclosan/earthworm/accumulation and distribution/enzyme activity分类
资源环境引用本文复制引用
沈海欣,冯永雪,马丽丽..聚乙烯颗粒对三氯生在蚯蚓体内富集的影响及其复合污染的毒性效应[J].南京师大学报（自然科学版）,2026,49(1):135-143,9.基金项目
国家自然科学基金项目(41603114)、江苏省科技厅自然科学基金项目(BK20161017). （41603114）
