农业生物技术学报2026，Vol.34Issue(3)：658-671,14.DOI:10.3969/j.issn.1674-7968.2026.03.017
马尾松冷休克蛋白(CSP)基因克隆及表达分析
Cloning and Expression Analysis of Cold Shock Protein(CSP)Gene in Pinus massoniana
摘要
Abstract
Cold shock protein(CSP)is an evolutionarily conserved DNA/RNA binding protein that plays a key role in the establishment of cold tolerance and growth,as well as in the development of plants.In order to understand the family characteristics of CSP gene(PmCSP)in Pinus massoniana,this study used full-length transcriptome data to identify the PmCSP gene family and to analyse its phylogeny,structure and functions,as well as the expression patterns of seedlings in response to cold stress.The results showed that 2 PmCSP gene family members were identified and cloned,both were intronless.The open reading frame of PmCSP1 was 642 bp in length,encoding 213 amino acids residues.The open reading frame of PmCSP2 was 603 bp in length,encoding 200 amino acids residues.The encoded polypeptide chains contained both cold shock domains and CCHC zinc finger domains,but differed in the number of zinc finger domains.PmCSP1 contained 3 domains,while PmCSP2 only contained 2 domains.Phylogenetic analysis showed that PmCSPs were more closely related to P.tabuliformis.Protoplast transient expression analysis showed that PmCSPs proteins were mainly located in the nucleus and cytoplasm.The expression of PmCSPs in the leaves and terminal buds of P.massoniana seedlings were higher than those in other tissues.Under cold stress,PmCSP1 and PmCSP2 were upregulated in the primary needle and secondary needle seedlings,respectively,with PmCSP expression peaking at 8 h,which indicated that PmCSPs were early response genes for P.massoniana seedlings in response to cold stress and may be involved in specific molecular regulation in heteroblastic foliage seedlings.This study provides a theoretical basis for further revealing the functions of the PmCSP gene family and the regulatory mechanisms established for the cold resistance of conifer heteroblastic foliage seedlings.关键词
马尾松幼苗/低温胁迫/异生叶/表达模式/CCHC锌指结构域Key words
Pinus massoniana seedlings/Cold stress/Heteroblastic foliage/Expression pattern/CCHC zinc finger domains分类
农业科技引用本文复制引用
李雪颖,徐盈盈,赵渊祥,吴峰..马尾松冷休克蛋白(CSP)基因克隆及表达分析[J].农业生物技术学报,2026,34(3):658-671,14.基金项目
贵州省重大科技专项(黔科合重大专项字[2024]010) （黔科合重大专项字[2024]010）
国家自然科学基金(32260406) （32260406）
