世界中医药2025，Vol.20Issue(20)：3591-3599,3608,10.DOI:10.3969/j.issn.1673-7202.2025.20.001
基于自噬的去腐生新膏治疗慢性创面的作用机制研究
Mechanisms of Qufu Shengxin Ointment in the Treatment of Chronic Wounds from the Perspective of Autophagy
摘要
Abstract
Objective:To explore the potential mechanisms of Qufu Shengxin Ointment in the treatment of chronic wounds(CW).Methods:Network pharmacology was used to analyze the common targets of the active components of the ointment,CW,and auto-phagy.Key signaling pathway factors with strong correlations were selected for animal experimental validation.A CW rat model was established,and wound healing was observed.Histopathological changes were examined using hematoxylin-eosin(HE)staining,and the expression levels of autophagy-related targets,including mammalian target of rapamycin(mTOR),hypoxia-inducible factor-1α(HIF-1α),and vascular endothelial growth factor(VEGF),were evaluated by immunohistochemistry and quantitative real-time PCR(qPCR).Results:Network pharmacology identified 40 core targets,including mTOR and HIF-1α.Enrichment analysis high-lighted the PI3K-AKT and HIF-1 signaling pathways,with mTOR closely associated with these pathways.HE staining showed that Qufu Shengxin Ointment reduced inflammatory cell infiltration and promoted angiogenesis.Immunohistochemistry results demonstra-ted that mTOR protein expression in the Qufu Shengxin Ointment group was significantly higher than in the Fujifu group at days 7 and 14 post-treatment(P＜0.05).HIF-1α protein expression at day 7 was higher than in both the model and Fujifu groups,while VEGF protein expression at day 14 was significantly higher than in these groups(P＜0.05).As revealed by qPCR results,mTOR mRNA levels at day 7 were significantly higher than in the model and Fujifu groups(P＜0.05).HIF-1α mRNA at day 7 was also significantly higher(P＜0.05).VEGF mRNA was significantly increased at day 7 compared to the model group,and at day 14,it was significantly higher than in both the model and Fujifu groups(P＜0.05).Conclusion:The external application of Qufu Shengxin Ointment may promote angiogenesis and accelerate CW healing,possibly through the regulation of autophagy-related path-way factors mTOR,HIF-1α,and VEGF.关键词
去腐生新膏/慢性创面/自噬/网络药理学/实验研究/作用机制Key words
Qufu Shengxin Ointment/Chronic wound/Autophagy/Network pharmacology/Experimental research/Mechanism of action分类
医药卫生引用本文复制引用
胡敏,陈海东,龚旭初,凌园园,方勇,杨万富,陈德轩,杨晋娴..基于自噬的去腐生新膏治疗慢性创面的作用机制研究[J].世界中医药,2025,20(20):3591-3599,3608,10.基金项目
第七批全国名老中医师承项目(国中医药人教函[2022]76号) （国中医药人教函[2022]76号）
江苏省中医药学会科研项目(PDJH2024051) （PDJH2024051）
江苏省南通市卫生健康委员会面上项目(MS2024042) （MS2024042）
江苏省中医流派研究院开放课题项目(JSZYLP2024050) （JSZYLP2024050）
江苏省中医流派研究院重点课题(LPZD2025009) （LPZD2025009）
南京中医药大学自然科学基金项目(XZR2021084) （XZR2021084）
