世界中医药2025，Vol.20Issue(20)：3624-3632,9.DOI:10.3969/j.issn.1673-7202.2025.20.005
经典名方温经汤基准样品关键质量属性研究
Critical Quality Attributes of Wenjing Decoction Benchmark Sample
摘要
Abstract
Objective:To elucidate the critical quality attributes(CQAs)of the benchmark sample of Wenjing Decoction and pro-vide a reference for its development and quality control.Methods:High-performance liquid chromatography(HPLC)was employed to generate fingerprint profiles of 15 batches of Wenjing Decoction using the Similarity Evaluation System for Chromatographic Fin-gerprint of Traditional Chinese Medicine(2012 edition),and similarities were evaluated to identify common peaks.The extract yield,moisture content,extractable matter,and multiple component content of the benchmark sample were determined.Hierarchical cluster analysis(HC A)was performed using SPSS 21.0,while principal component analysis(PC A)and orthogonal partial least squares-discriminant analysis(OPLS-DA)were conducted using SIMCA 14.0 to assess quality differences among the 15 batches.Variable importance in projection(VIP)values were used to identify key marker compounds influencing quality.Results:A total of 20 common peaks were identified across the 15 batches of Wenjing Decoction.The extract yield ranged from 20.76％to 24.27％;moisture content from 5.25％to 7.51％;and extractable matter from 48.10％to 53.99％.The content of major components(mg/g)was as follows:gallic acid(0.28 to 0.39),paeoniflorin(0.45 to 0.55),liquiritin(0.18 to 1.13),ferulic acid(0.05 to 0.34),cinnamic acid(0.02 to 0.04),cinnamaldehyde(0.07 to 0.22),paeonol(0.41 to 0.61),ammonium glycyrrhizinate(1.31 to 2.72),ginsenoside Rg1(0.80 to 1.18),ginsenoside Re(0.51 to 0.76),and ginsenoside Rb1(0.90 to 1.55).Conclu-sion:The study comprehensively characterized the CQAs of Wenjing Decoction,providing a scientific basis for its formulation devel-opment and quality control.关键词
经典名方/温经汤/基准样品/指纹图谱/含量测定/聚类分析/主成分分析/正交偏最小二乘判别法分析Key words
Classical famous prescription/Wenjing Decoction/Benchmark sample/Fingerprint/Content determination/Cluster anal-ysis/Principal component analysis/Orthogonal partial least squares-discriminant analysis分类
医药卫生引用本文复制引用
李军鸽,邱智东,刘晓星,王锐,王永春,王新蕊,唐秋竹..经典名方温经汤基准样品关键质量属性研究[J].世界中医药,2025,20(20):3624-3632,9.基金项目
国家自然科学基金青年科学基金项目(82404842) （82404842）
吉林省科技发展计划项目(20230204037YY) （20230204037YY）
