世界中医药2025，Vol.20Issue(20)：3693-3700,8.DOI:10.3969/j.issn.1673-7202.2025.20.013
柴胡-浙贝母药对及其活性成分治疗乳腺癌癌前病变大鼠的作用机制
Therapeutic Effects of Bupleuri Radix-Fritillariae Thunbergii Bulbus Herbal Pair and Its Active Components on Preneoplastic Breast Cancer in Rats
摘要
Abstract
Objective:To investigate the effects and mechanisms of the Bupleuri Radix-Fritillariae Thunbergii Bulbus herbal pair and its active components on preneoplastic breast cancer(PBC)in rats,focusing on angiogenesis mediated by the signal transducer and activator of transcription 3(STAT3)/vascular endothelial growth factor(VEGF)signaling pathway.Methods:Forty-five female Sprague Dawley(SD)rats were randomly assigned to five groups using a random number table:blank control,model,tamoxifen(TAM),Bupleuri Radix-Fritillariae Thunbergii Bulbus granules(granules),and saikosaponin D-peimine(monomer).Except for the blank group,the PBC model was established in the other groups by sequential administration of 7,12-dimethylbenz[a]anthracene(DMBA),estrogen,and progesterone.After successful modeling,the corresponding drugs were administered for 4 weeks.Therapeu-tic effects were evaluated by uterine and ovarian coefficients,hematoxylin-eosin(HE)staining,immunohistochemistry,quantitative real-time polymerase chain reaction(qPCR),and Western blot(WB)analyses.Results:Compared with the blank group,the model group showed severe ductal lesions,epithelial cell stacking,unclear ductal structures,and pronounced hyperplasia,confirming suc-cessful modeling.Compared with the model group,TAM,granules,and monomer groups all showed improvement in these pathologi-cal features,with reductions in uterine and ovarian coefficients and levels of VEGF-A,STAT3,basic fibroblast growth factor(bF-GF),and interleukin-6(IL-6)(P＜0.01,P＜0.05).Additionally,TAM and granules groups exhibited decreased microvessel den-sity(CD31-MVD)and phosphorylated STAT3(p-STAT3)levels(P＜0.01,P＜0.05).Conclusion:The Bupleuri Radix-Fritillari-ae Thunbergii Bulbus herbal pair and its active components may exert therapeutic effects on PBC by inhibiting STAT3/VEGF path-way-mediated angiogenesis.关键词
乳腺癌癌前病变/信号转导和转录激活因子3/血管内皮生长因子信号通路/血管生成/柴胡-浙贝母药对/柴胡皂苷D-贝母甲素/活性成分/乳腺癌/大鼠分类
医药卫生引用本文复制引用
徐留燕,程旭锋,张文可,刘琦,赵慧朵..柴胡-浙贝母药对及其活性成分治疗乳腺癌癌前病变大鼠的作用机制[J].世界中医药,2025,20(20):3693-3700,8.基金项目
全国名老中医药专家传承工作室建设项目(2100601-CZ0175) （2100601-CZ0175）
2022年河南省科技攻关计划项目(222102310326) （222102310326）
河南省中医药重点学科(中医外科)建设项目(CZ0366-07) （中医外科）
2023年度河南省自然科学基金面上项目(232300421183) （232300421183）
