首都师范大学学报（自然科学版）2026，Vol.47Issue(1)：64-70,7.DOI:10.19789/j.1004-9398.2026.01.008
HPLC-MS/MS快速测定鸡肉中酰胺醇类和硝基咪唑类药物
Determination chloramphenicol and nitroimidazole residues in chicken by HPLC-MS/MS
摘要
Abstract
To achieve the rapid and simultaneous determination of amide alcohol and nitroimidazole drug residues in chicken,a high-performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS)analytical detection technique was established in this paper.The chicken samples were homogenized and crushed,pre-mixed and dispersed with water,extracted with acetonitrile,purified in one step with a Captiva EMR-Lipid solid-phase extraction column,and determined by HPLC-MS/MS.The amide alcohol and nitroimidazole drugs were quantified by the matrix-matched standard solution internal standard method and the matrix-matched standard solution external standard method,respectively.The amide alcohol and nitroimidazole drugs showed good linear relationships within the concentration range of 0.3 to 10.0 µg/kg.The detection limits were both 0.1 µg/kg,and the quantification limits were both 0.3 µg/kg.At the levels of 1.0、2.0 and 5.0 µg/kg,the recovery rates of the amide alcohol drugs ranged from 95.5%to 105.8%,and the recovery rates of the nitroimidazole drugs ranged from 82.4%to 109.3%,with relative standard deviations ranging from 0.4%to 10.1%.This method is simple and rapid,and meets the relevant regulations and requirements for the detection of amide alcohol and nitroimidazole drug residues in chicken.关键词
鸡肉/酰胺醇类/硝基咪唑类/HPLC-MS/MSKey words
chicken/chloramphenicol/nitroimidazole/HPLC-MS/MS分类
轻工纺织引用本文复制引用
李洪波,黄志伟,张振宇,刘萌,马宁..HPLC-MS/MS快速测定鸡肉中酰胺醇类和硝基咪唑类药物[J].首都师范大学学报（自然科学版）,2026,47(1):64-70,7.基金项目
河南省济源市科技攻关项目(24021001,24021006) （24021001,24021006）
