世界中医药2025，Vol.20Issue(20)：3721-3730,10.DOI:10.3969/j.issn.1673-7202.2025.20.017
电针对高血压大鼠肠道菌群和p38丝裂原活化蛋白激酶通路的影响
Effects of Electroacupuncture on Intestinal Flora and P38 MAPK Pathway in Hypertensive Rats
摘要
Abstract
Objective:To investigate the effects of electroacupuncture on blood pressure,intestinal flora,ghrelin,and the p38 mito-gen-activated protein kinase/endothelial nitric oxide synthase(p38 MAPK/eNOS)signaling pathway in spontaneously hypertensive rats(SHRs),aiming to elucidate the antihypertensive mechanism of electroacupuncture and provide a scientific basis for its use in treating hypertension.Methods:Sixteen SHRs were randomly divided into a model group and an electroacupuncture group using a random number table,and eight Wistar-Kyoto(WKY)rats were included as a blank group.Rats in the electroacupuncture group re-ceived electroacupuncture(continuous wave,2 Hz,1 to 2 mA)for 20 min per day,5 times per week,for 4 weeks.Rats in the blank and model groups were grasped and immobilized for 20 min at the same time each day,5 times per week,for 4 weeks.Systolic blood pressure(SBP)and diastolic blood pressure(DBP)were measured using the tail-cuff method before and after the intervention.Fe-cal microbial communities were analyzed by 16S rDNA sequencing.Serum levels of ghrelin,thromboxane A2(TXA2),and nitric ox-ide(NO)were measured using enzyme-linked immunosorbent assay(ELISA).The expression of p38 MAPK and eNOS in vascular tissues was detected using quantitative real-time polymerase chain reaction(qRT-PCR).Results:Electroacupuncture significantly improved SBP and DBP in SHRs(both P＜0.01).Compared with the blank group,the number of operational taxonomic units(OTUs)decreased in the model group;compared with the model group,the number of OTUs increased significantly in the electroa-cupuncture group(P＜0.05),and the Shannon index was upregulated(P＜0.05).Linear discriminant analysis effect size(LEfSe)showed that Lachnospiraceae_NK4A136_group,Ruminococcaceae_UCG_013,and Clostridiales were significantly enriched in the electroacupuncture group,whereas Allobaculum was significantly enriched in the model group.Correlation analysis indicated that Akkermansia(R=-0.592,P=0.02)and Clostridium_sensu_stricto_1(R=-0.515,P=0.049)were negatively correlated with DBP,and Prevotellaceae_UCG-001 was positively correlated with DBP(R=0.531,P=0.042).In addition,electroacupuncture in-creased serum ghrelin and NO levels and decreased TXA2 levels(both P＜0.01).There was a trend toward decreased p38 MAPK expression and increased eNOS expression in the electroacupuncture group.Conclusion:Electroacupuncture effectively reduced SBP and DBP in SHRs,increased OTU numbers and microbial diversity in the intestinal flora,upregulated ghrelin,eNOS,and NO levels,decreased TXA2 levels,and downregulated p38 MAPK expression.The antihypertensive effect of electroacupuncture may be related to regulation of intestinal flora,ghrelin,and the p38 MAPK/eNOS signaling pathway.关键词
电针/自发性高血压大鼠/肠道菌群/p38丝裂原活化蛋白激酶/内皮型一氧化氮合酶信号通路/内皮功能障碍Key words
Electroacupuncture/Spontaneously hypertensive rats/Intestinal flora/P38 MAPK/eNOS signaling pathway/Endothe-lial dysfunction分类
医药卫生引用本文复制引用
李涓,李惠菁,张月,梁倩,江一静,罗霄,金荣疆,张丽丽,钟冬灵..电针对高血压大鼠肠道菌群和p38丝裂原活化蛋白激酶通路的影响[J].世界中医药,2025,20(20):3721-3730,10.基金项目
国家自然科学基金面上项目(82074516)——基于RVLM区RAS双轴及其下游通路介导的神经炎症与交感神经兴奋探讨从阳明论治高血压的中枢机制 （82074516）
国家自然科学基金面上项目(82474618)——基于NTS区GABA神经元E/I平衡调控NTS-CVLM-RVLM神经环路介导的自主神经稳态探讨从阳明论治高血压的中枢机制 （82474618）
四川省科学技术厅创新人才项目(2023JDRC0026)——基于"肠道菌群-LPS-TLR4"途径介导的神经炎症驱动高血压交感神经兴奋探讨电针阳明经穴的降压机制 （2023JDRC0026）
