世界科学技术-中医药现代化2026，Vol.28Issue(1)：153-163,11.DOI:10.11842/wst.20250303001
活血潜阳祛痰方抑制铁死亡改善肥胖高血压大鼠心肌重构的研究
Inhibition of Ferroptosis and Improvement of Myocardial Remodeling in Obese Hypertensive Rats by Huoxue Qianyang Qutan Recipe
摘要
Abstract
Objective To evaluate the efficacy of Huoxue Qianyang Qutan Recipe(HQQR),a clinically proven traditional Chinese medicine formula,in attenuating myocardial remodeling in obesity hypertension(OBH)rats by inhibiting ferroptosis.Methods A rat model of OBH was established by feeding spontaneously hypertensive rats(SHR)by a high-fat diet.Successfully modeled OBH rats were randomly divided into the model group(OBH),low-dose HQQR group(HQQR-L,19.35 g crude drug/kg/d),high-dose HQQR group(HQQR-H,38.7 g crude drug/kg/d),and valsartan group(30 mg/kg/d).Age-matched SHR and Wistar Kyoto rats fed a standard diet served as controls.After 10 weeks of drug intervention,body weight,Lee's index,liver index,blood pressure,glucose and lipid metabolism parameters,and indices of myocardial remodeling were measured.Myocardial pathological changes were observed by HE and Masson's trichrome staining.Biochemical assays were used to detect myocardial levels of ferrous ion(Fe²⁺)and glutathione(GSH).Additionally,the mRNA and protein expression levels of ferroptosis-related markers,solute carrier family 7 member 11(SLC7A11)and glutathione peroxidase 4(GPX4),were measured by RT-qPCR and Western blot,respectively.Results OBH rats exhibited typical symptoms of obesity,hypertension,metabolic dysregulation,and significant myocardial remodeling,characterized by cardiomyocyte edema and extensive fibrosis.Fe²⁺ accumulation markedly increased in myocardial tissue,while levels of GSH,SLC7A11,and GPX4 significantly reduced.Treatment with HQQR significantly decreased body weight,Lee's index,liver index,and blood pressure(P<0.05),improved glucose and lipid metabolism(P<0.05),alleviated myocardial remodeling(P<0.05),reduced myocardial fibrosis,and ameliorated cardiomyocyte morphology(P<0.05).Furthermore,HQQR significantly reduced Fe²⁺ levels(P<0.05),increased GSH levels(P<0.05),and upregulated the mRNA and protein expression of SLC7A11 and GPX4 in a dose-dependent manner(P<0.05).Conclusion HQQR may ameliorate myocardial remodeling in OBH rats by inhibiting ferroptosis,likely through the activation of the SLC7A11/GPX4 pathway.关键词
活血潜阳祛痰方/肥胖高血压/铁死亡/心肌重构Key words
Huoxue Qianyang Qutan recipe/Obesity hypertension/Ferroptosis/Myocardial remodeling分类
医药卫生引用本文复制引用
赵妤修,马玉龙,芦波,李达,陈晓喆,王明珠,符德玉..活血潜阳祛痰方抑制铁死亡改善肥胖高血压大鼠心肌重构的研究[J].世界科学技术-中医药现代化,2026,28(1):153-163,11.基金项目
国家自然科学基金委员会面上项目(82174130):基于线粒体自噬与NLRP3炎性小体crosstalk探讨活血潜阳祛痰方改善OBH心肌损害的机制研究,负责人:符德玉 （82174130）
国家自然科学基金委员会面上项目(82274262):活血潜阳祛痰方调控FUNDC1-MAM-线粒体Ca2+稳态改善OBH心肌重构的机制研究,负责人:芦波 （82274262）
上海市白玉兰人才计划浦江项目(24PJD113):基于线粒体内质网Ca2+转运介导的铁死亡探讨活血潜阳祛痰方对OBH心肌重构的干预机制,负责人:芦波. （24PJD113）
