饲料工业2026，Vol.47Issue(3)：86-92,7.DOI:10.13302/j.cnki.fi.2026.03.012
基于网络药理学探讨黄芩苷缓解AFB1诱导肝细胞凋亡的靶点和通路
Exploring Targets and Pathways of Baicalin to Alleviate AFB1-Induced Apoptosis in Hepatocytes Based on Network Pharmacology
摘要
Abstract
The aim of this study was to investigate the target and pathway of baicalin in alleviating AFB1-induced hepatocyte apoptosis by using network pharmacology.Firstly,the targets of baicalin,AFB1 and hepatocyte apoptosis were summarized by PharmMapper database,SwissTargetPrediction database,TCMSP database and Metascape data-base to build venn diagram.Secondly,PPI interac-tive network is constructed using Cytoscape 3.7.2 software.Then,the Metascape database is used for the key target for gene ontology(GO)function annotation and kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis.Finally,the ability to analyze the combination of baicalin and core targets through molecular docking technology.Based on Pharmmapper,Swiss Target Prediction,TCMSP database obtained 370 targets of baicalin,based on Genecards and OMIM databases,148 targets of liver injury caused by AFB1.Through Venn analysis,there are 17 common inter-section targets to obtain baicalin and AFB1.Use the String database to build a PPI interactive network,and combine the Cytoscape software analysis to further screen the key target of baicalin to relieve AFB1.There are 81 targets for baicalin,51 targets of liver injury caused by AFB1,and 14 intersection targets,namely JNK1,GPT,TLR4,HPRT1,CYP1A1,HMOX1,BAX,TP53,PTGS2,Caspase3,MAPK1,EGFR,TGFB1,CYP1A2.GO enrichment analysis showed that baicalin alleviated AFB1-induced hepatocyte apoptosis mainly involved oxidative stress,mitochondrial damage and other related biological processes;KEGG enrichment analysis showed that Mitogen-Activated Protein Kinase(MAPK)signaling pathway played a major role in baicalin alleviating AFB1 induced hepatocyte apoptosis.Baicalin has the potential to target JNK1,TGFB1,EGFR,MAPK1,BAX and other targets.Based on the results of network pharmacology,this study discussed that baicalin may participate in MAPK and other related signaling pathways by targeting JNK1,GPT,TLR4,HPRT1,CYP1A1,HMOX1,BAX,TP53,PTGS2,Caspase3,MAPK1,EGFR,TGFB1,CYP1A2 related targets,and play a role in alleviating AFB1 induced hepatocyte apoptosis.关键词
黄芩苷/肝细胞凋亡/AFB1/网络药理学/靶点Key words
baicalin/hepatocyte apoptosis/AFB1/network pharmacology/target分类
农业科技引用本文复制引用
朱意,闻德锋,周华林,邱银生,熊江林,吴仲元,陆启荣..基于网络药理学探讨黄芩苷缓解AFB1诱导肝细胞凋亡的靶点和通路[J].饲料工业,2026,47(3):86-92,7.基金项目
湖北省重点研发计划项目[2023BBB069] （）
动物营养与饲料科学湖北省重点实验室开放课题项目[202313] （）
