生物技术通报2026，Vol.42Issue(1)：105-113,9.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0833
文心兰'金辉'和'香水文心'花香形成比较分析
Comparative Analysis of Floral Scent Formation between'Jinhui'and Onc.'Sharry Baby'of Oncidium hybridum
摘要
Abstract
[Objective]A new strain'Jinhui'was bred from tissue culture seedlings of Oncidium Gower Ramsey,which was tall and upright,but had no aroma.The aim of this study is to explore the non-aroma limiting factors of'Jinhui'by analyzing molecular mechanism of floral scent formation involved in unscented'Jinhui'and strong scented Onc.'Sharry Baby',aiming to release aroma of'Jinhui'by molecular technology.[Method]HS-SPME/GC-MS was used to determine components and contents of floral volatiles from two varieties of Oncidium hybridum.Key genes of floral scent formation were screened by transcriptome sequencing and qRT-PCR using different flower developmental stages of'Jinhui'and Onc.'Sharry Baby'as materials.[Result]GC-MS results showed that total floral volatile contents of blooming period in Onc.'Sharry Baby'were 6.1 times higher than that of'Jinhui'.The main floral volatiles in Onc.'Sharry Baby'was monoterpenes,while it was sesquiterpenes in'Jinhui'.The results of transcriptome sequencing showed that 55 277 unigenes were obtained,and about 81.64%unigenes were annotated by public database.In MEP pathway,the upstream pathway of monoterpenes,most of the genes,such as CMK,HDR,HDS and GPPS,were expressed higher in Onc.'Sharry Baby'than that in'Jinhui',thus that GPP,precursor of monoterpenes,was accumulated in the Onc.'Sharry Baby'.In MVA pathway,the upstream pathway of sesquiterpene,about half of genes were highly expressed in Onc.'Sharry Baby',and half of them were highly expressed in'Jinhui'.Three terpene synthase genes(TPS)with significant differential expression were screened from transcriptome data.Among them,OhTPS1 and OhTPS2 belonged to the TPS-b family and were mainly composed of mono-TPSs genes.The expressions of OhTPS1 and OhTPS2 in Onc.'Sharry Baby'were significantly higher than that in'Jinhui'and increased gradually with flower development in Onc.'Sharry Baby',indicating that these two genes played a key role in monoterpene biosynthesis of Onc.'Sharry Baby'.OhTPS3 belonged to TPS-a family and were mainly composed of sesqui-TPSs genes.The expression of OhTPS3 in'Jinhui'was significantly higher than that in Onc.'Sharry Baby'and increased gradually with flower development in'Jinhui',indicating that this gene played a key role in sesquiterpenoid biosynthesis of'Jinhui'.[Conclusion]The main reason for low total amount of volatile compounds in'Jinhui'flowers is that most of key genes in MEP pathway are low expression,which limits the accumulation of GPP,and low expression of monoterpene synthase genes also hinder the formation of monoterpene volatiles.关键词
文心兰/花香/转录组/萜类化合物Key words
Oncidium hybridum/floral scent/transcriptome/terpenoids引用本文复制引用
樊荣辉,罗远华,陈艺荃,方能炎,陈燕,钟淮钦,叶秀仙..文心兰'金辉'和'香水文心'花香形成比较分析[J].生物技术通报,2026,42(1):105-113,9.基金项目
福建省自然科学基金项目(2023J01198),院青年英才项目(YC2021018),福建省人民政府与中国农业科学院高质量发展超越"5511"协同创新工程项目(XTCXGC2021003),省级财政花卉产业发展项目(闽林文[2023]107号) （2023J01198）
