生物技术通报2026，Vol.42Issue(1)：198-207,10.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0901
花生转录因子AhHDZ70的生物信息学分析及耐盐耐旱性研究
Bioinformatics Analysis of Peanut Transcription Factor AhHDZ70 and Its Tolerances to Salt and Drought
摘要
Abstract
[Objective]Drought and salinity stress are critical environmental factors that severely affect the yield and quality of peanuts(Arachis hypogaea L.)in China.Investigating the structure and function of AhHDZ70(Ah12g074600.1)gene will reveal its function in tolerance of peanut to drought and salt,and provide essential gene resources for peanut salt resistance and drought resistance breeding.[Method]Bioinformatics method were applied to analyze the physical and chemical properties,gene structure,phylogeny,cis-element of promoter,protein interaction of AhHDZ70.Fluorescence quantitative PCR technology was used to identify the expressions of AhHDZ70 gene in different tissues and organs,and under salt,drought,high temperature and low temperature stresses.Subcellular localization analysis was performed using tobacco transient transformation technology.Genetic transformation was carried out in Arabidopsis and biological function of AhHDZ70 was further verified by salt and drought stress treatment.[Result]AhHDZ70 encoded 326 amino acids,which was an unstable hydrophilic protein with no signal peptide,its secondary and tertiary structures were mainly characterized by irregular curling.Domain analysis showed that AhHDZ70 contained 4 exons and 3 introns.The AhHDZ70 promoter region contained two drought-induced functional elements(MBS motif),one adversity stress response element(TC-richrepeats motif),and one cryogenic response element(LTR motif).The main form of its secondary structure and tertiary structure was irregular curl.Transcriptome combined with RT-qPCR analysis showed that AhHDZ70 had the highest expression in seeds and responded positively to salt,drought,high temperature and low temperature stress.The results of subcellular localization showed that AhHDZ70 was located in the nucleus.AhHDZ70 heterologously overexpressed Arabidopsis to obtain homozygous transgenic plants.Under salt and drought stress,the germination rate of AhHDZ70-overexpressed transgenic lines was significantly higher than that of WT,and the root length was significantly longer than WT.[Conclusion]AhHDZ70 belongs to the HD-ZIP II transcription factor and is located in the nucleus.AhHDZ70 might play a regulatory role in the growth and development of various tissues in peanuts.Additionally,AhHDZ70 is likely involved in positively regulating peanut's response to salt and drought stress.关键词
花生/转录因子/AhHDZ70/生物信息学分析/表达模式/耐盐/耐旱/基因功能Key words
peanut/transcription factor/AhHDZ70/bioinformatics analysis/expression pattern/tolerance to salt/tolerance to drought/gene function引用本文复制引用
吴翠翠,陈登科,兰刚,夏芝,李朋波..花生转录因子AhHDZ70的生物信息学分析及耐盐耐旱性研究[J].生物技术通报,2026,42(1):198-207,10.基金项目
山西省科技重大专项"揭榜挂帅"项目(202201140601025-4-03),山西农业大学棉花研究所博士基金项目(SBSJJ2023-02),山西农业大学科技创新提升工程项目(CXGC2023054) （202201140601025-4-03）
