天津中医药2026，Vol.43Issue(2)：205-211,7.DOI:10.11656/j.issn.1672-1519.2026.02.10
宣肺败毒方中药单体通过IL-6/IL-6R调控的抗炎作用筛选研究
Screening of anti-inflammatory monomeric compounds from Xuanfei Baidu Decoction via regulation of the IL-6/IL-6R pathway
摘要
Abstract
[Objective]To screen the Chinese herbal monomer components from Xuanfei Baidu Formula(XFBD)with anti-inflammatory activity based on molecular docking and competitive enzyme-linked immunosorbent assay(ELISA)experiments.[Methods]Using Discovery Studio 2.5,molecular docking was performed between 963 herbal monomer components contained in XFBD and interleukin-6 receptor(IL-6R).A competitive ELISA-based screening model for IL-6/IL-6R antagonists was established to validate the inhibitory effects of monomer components on IL-6/IL-6R binding.[Results]Molecular docking results indicated that 19 monomer components from XFBD exhibited strong binding potential to IL-6R.Competitive ELISA results showed that the optimal binding concentration between IL-6R and IL-6 was 2 μg/mL,under which the IL-6/IL-6R antagonist screening model was constructed.Using this model,the inhibitory activity of the 19 monomer components was verified.The results demonstrated that all 19 components significantly inhibited the binding of IL-6 to IL-6R.Among them,eight components-hesperidin,amygdalin,isorhamnetin-3-O-rutinoside,vitexin,loganin,stearic acid,kaempferol,and p-methoxycinnamic acid-showed inhibition rates exceeding 50%.[Conclusion]Multiple monomer components in Xuanfei Baidu Formula exert anti-inflammatory effects by blocking IL-6/IL-6R binding,providing a material basis for its anti-inflammatory activity.关键词
宣肺败毒方/白细胞介素-6/白细胞介素-6受体拮抗剂/中药单体/分子对接/竞争性酶联免疫吸附测定/抗炎Key words
Xuanfei Baidu Decoction/IL-6/IL-6R antagonist/Chinese medicine monomers/molecular docking/competitive enzyme-linked immunosorbent assay/anti-inflammatory分类
医药卫生引用本文复制引用
常惠琳,罗子娟,李舒宁,高盼微,王萌,袁庆,苗琳,张晗,柴丽娟..宣肺败毒方中药单体通过IL-6/IL-6R调控的抗炎作用筛选研究[J].天津中医药,2026,43(2):205-211,7.基金项目
天津市科技计划项目(22ZXGBSY00020) （22ZXGBSY00020）
现代中药新质生产力科技创新工程专项(24ZXZKSY00010). （24ZXZKSY00010）
