天津中医药2026，Vol.43Issue(2)：218-223,6.DOI:10.11656/j.issn.1672-1519.2026.02.12
基于NMDA通路探讨茸菖胶囊干预月经性癫痫神经元模型的抗痫机制
Anti-epileptic mechanism of Rongchang Capsule intervention in the neuronal model of catamenial epilepsy was discussed based on the NMDA pathway
摘要
Abstract
[Objective]To observe the effect of Rongchang Capsules on N-Methyl-D-aspartic acid(NMDA)receptor in the neuronal model of catamenial epilepsy,and to explore its mechanism.[Methods]The magnesium-free epileptic neurons induced by exogenous E2(catamenial epileptic neuron model)were randomly divided into 7 groups:control group,epilepsy model group,catamenial epilepsy model group,sodium valproate group and Rongchang Capsules low dose group,Rongchang Capsules middle dose group and Rongchang Capsules high dose group.The neuronal morphology,discharge frequency,resting membrane potential,24 h NMDA current changes,NMDA Receptor 1(NR1)and NMDA Receptor 2A(NR2A)receptor protein expression were observed.[Results]Compared with the control group,the high-frequency and high-amplitude spike discharges in the catamenial epilepsy model group were significantly increased;the number of neurons was significantly decreased;the discharge frequency was significantly increased;the resting membrane potential was significantly increased;the 24 h NMDA channel current density was significantly increased(P<0.05 or P<0.01);the expression of NR1 protein was significantly decreased,and the expression of NR2A protein was significantly increased(P<0.05 or P<0.01).Compared with the catamenial epilepsy model group,the high-frequency high-amplitude spike wave discharge in the sodium valproate group was significantly decreased;the number of neurons increased;the discharge frequency decreased significantly(P<0.05);the resting membrane potential and the 24 h NMDA channel current density was not decreased significantly(P>0.05);the expression of NR1 and NR2A protein did not change significantly(P>0.05);the high-frequency and high-amplitude spike wave discharge in the Rongchang capsules high dose group was significantly decreased;the number of neurons was significantly increased;the discharge frequency was significantly decreased;the resting membrane potential was significantly decreased and the 24 h NMDA channel current density was significantly decreased(P<0.05),and the expression of NR2A protein was significantly decreased(P<0.05),and the expression of NR1 protein was not significantly increased(P>0.05).[Conclusion]Rongchang Capsules may reduce the abnormal excitability of NMDA receptor-mediated nerves by down-regulating the expression of NR2A protein,thereby reducing the abnormal discharge of magnesium-free epileptic neurons supplemented with exogenous E2,thus exerting anti-epileptic effects.关键词
茸菖胶囊/月经性癫痫/神经元模型/NMDA通路/抗痫机制Key words
Rongchang Capsule/catamenial epilepsy/neuronal model of catamenial epileptic/NMDA pathway/antiepileptic mechanism分类
医药卫生引用本文复制引用
闫海虹,郭婷,陈海鹏,张喜莲,戎萍,李瑞本,付乾芳,马融..基于NMDA通路探讨茸菖胶囊干预月经性癫痫神经元模型的抗痫机制[J].天津中医药,2026,43(2):218-223,6.基金项目
国家自然科学基金项目(81574018) （81574018）
山西省基础研究计划项目(202403021222477). （202403021222477）
