天津中医药2026，Vol.43Issue(2)：231-237,7.DOI:10.11656/j.issn.1672-1519.2026.02.14
苓桂术甘汤调控PINK1/Parkin通路改善线粒体自噬对非酒精性脂肪肝炎小鼠的影响
Effect of Linggui Zhugan Decoction in regulating PINK1/Parkin pathway to improve mitophagy in the NASH mice
摘要
Abstract
[Objective]To investigate the therapeutic effect of Linggui Zhugan Decoction(LGZG)on non-alcoholic steatohepatitis(NASH)model mice and its impact on the PINK1/Parkin signaling pathway.[Methods]A NASH mouse model was established using a methionine-and choline-deficient(MCD)diet,followed by intragastric administration of different doses of LGZG.The therapeutic effect of LGZG on NASH model mice was assessed by measuring body weight,liver index,serum ALT,AST,TG,and TC levels,as well as by performing hematoxylin-eosin(HE)staining and Oil Red O staining on frozen liver tissues.The liver's antioxidant capacity was evaluated by detecting levels of superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px),and reactive oxygen species(ROS).To explore the effect of LGZG on mitophagy,the protein levels of mitochondrial markers(TOM20,COXⅣ,VDAC1)and key proteins of the PINK1/Parkin pathway(PINK1,Parkin,LC3-Ⅱ,P62,Beclin1)were measured.[Results]LGZG significantly increased the body weight of NASH model mice;reduced the liver index;decreased serum ALT and AST activities;lowered TC and TG levels;and ameliorated pathological changes in the liver tissue of NASH model mice.These results indicate that LGZG has a therapeutic effect on NASH model mice.LGZG increased SOD and GSH-Px levels while decreasing MDA and ROS levels,suggesting enhanced hepatic antioxidant capacity.Furthermore,LGZG increased the protein levels of PINK1,Parkin,LC3-Ⅱ,and Beclin1,while decreasing the protein levels of TOM20,COXⅣ,VDAC1,and P62.This demonstrates that LGZG improved PINK1/Parkin-mediated mitophagy in NASH mice.[Conclusion]LGZG exhibits a therapeutic effect on NASH model mice and enhances hepatic antioxidant capacity.The underlying mechanism may be associated with the regulation of the PINK1/Parkin signaling pathway to improve mitophagy.关键词
苓桂术甘汤/非酒精性脂肪肝炎/线粒体自噬/氧化应激/PINK1/Parkin通路Key words
Linggui Zhugan Decoction/non-alcoholic steatohepatitis/mitochondrial autophagy/oxidative stress/PINK1/Parkin pathway分类
医药卫生引用本文复制引用
武锐,张辉,司御臣,王蕊,李玉玲,徐凯,范立荣,田甜,吕树泉,郭煊..苓桂术甘汤调控PINK1/Parkin通路改善线粒体自噬对非酒精性脂肪肝炎小鼠的影响[J].天津中医药,2026,43(2):231-237,7.基金项目
苏秀海全国名老中医药专家传承工作室(国中医药人教函[2022]75号). （国中医药人教函[2022]75号）
